Quantitative Detection of Trichloroethylene-Degrading Mycobacterium sp. TA27 with a Real-time PCR Product Detection System
スポンサーリンク
概要
- 論文の詳細を見る
We developed a method for enumerating trichloroethylene (TCE) degrading Mycobacterium sp. TA27 by means of PCR. The PCR primers and probe were designed based on the 16S rRNA sequences of Mycobacterium sp. TA27, compared with corresponding regions of 8 phylogenetically related strains. The total DNAs of strain TA27 and the other 8 mycobacteria were extracted and used for PCR amplification by a 5'-nuclease real-time PCR assay. The highest specificity for strain TA27 was observed at an annealing temperature of 62℃ and at forward and reverse primer concentrations of 0.2 pM each. The other 8 mycobacteria were not detected under these conditions. The detection limits for the DNA and cells of strain TA27 were 0.05 pg/tube and 25 cells/tube, respectively. The utility of the PCR assay for the quantification of strain TA27 cells was demonstrated in TCE-polluted groundwater.
- 日本微生物生態学会の論文
著者
-
Yagi Osami
Water And Soil Environmental Studies
-
IWASAKI KAZUHIRO
National Institute for Environmental Studies
-
Hashimoto Akiko
National Institute For Environmental Studies And Crest Japan Science And Technology Corporation
-
Iwasaki K
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat
-
Yagi O
Univ. Tokyo Tokyo Jpn
-
Yagi Osami
Research Center For Water Environment Technology Graduate School Of Engineering The University Of To
-
NAKAJIMA Mutsuyasu
College of Bioresource Sciences, Nihon University
-
Iwasaki K
National Inst. For Environmental Studies Ibaraki Jpn
-
Nakajima M
College Of Bioresource Sciences Nihon University
関連論文
- Recovery of ^Cs by a Bioaccumulation System Using Rhodococcus erythropolis CS98
- Kinetic Studies on Cesium Transport in Rhodococcus erythropolis CS98 and Rhodococcus sp. Strain CS402
- Trichloroethylene Degradation by Cells of a Methane-Utilizing Bacterium, Methylocystis sp. M, Immobilized in Calcium Alginate
- On the Interaction of Ribosomal Protein L5 with 5S rRNA(Biochemistry & Molecular Biology)
- Requirement for C-terminal Extension to the RNA Binding Domain for Efficient RNA Binding by Ribosomal Protein L2(Biochemistry & Molecular Biology)
- Purification and Properties of a Soluble Methane Monooxygenase from Methylocystis sp. M
- Novel Metabolite of Trichloroethylene in a Methanotrophic Bacterium, Methylocystis sp. M, and Hypothetical Degradation Pathway
- Protein Phosphatase Inhivition Assay for Detection of Microcystins in Lake Water and Microcystis Cultures
- Random Amplified Polymorphic DNA (RAPD) Analyses for Discriminating Genotypes of Microcystis Cyanobacteria
- Analysis of Gene Expression in Yeast Protoplasts Using DNA Microarrays and Their Application for Efficient Production of Invertase and α-Glucosidase (BIOCHEMICAL ENGINEERING)
- Growth Inhibition of Microcystis Cyanobacteria by L-Lysine and Disappearance of Natural Microcystis Blooms with Spraying
- Aerobic Degradation of Trichloroethylene by a New Type II Methane-Utilizing Bacterium, Strain M(Microbiology & Fermentation Industry)
- Aerobic Degradation of Trichloroethylene at High Concentration by a Methane-Utilizing Mixed Culture(Microbiology & Fermentation Industry)
- Development of a Most-probable-number Method for Enumerating Denitrifying Bacteria by Using 96-Well Microtiter Plates and an Anaerobic Culture System
- Development of a New Most-probable-number Method for Enumerating Methanotrophs, Using 48-well Microtiter Plates
- Characteristics of Escherichia coli HB101 and Pseudomonas putida PpY101 Harboring a Recombinant Plasmid with Tandem Insertion of the Mercury Resistance Operon
- Immobilization of Trichloroethylene-Degrading Bacterium, Methylocystis sp. Strain M in Different Matrices
- Isolation and Characterization of the Slime from a Cyanobacterium, Microcystis aeruginosa K-3A(Microbiology & Fermentation Industry)
- Degradation Pathways of Trichloroethylene and 1,1,1-Trichloroethane by Mycobacterium sp. TA27(Microbiology & Fermentation Technology)
- Quantitative and Specific Detection of a Trichloroethylene-degrading Methanotroph, Methylocystis sp. Strain M, by a Most Probable Number-Polymerase Chain Reaction Method(Microbiology & Fermentation Technology)
- Quantitative Detection of Trichloroethylene-Degrading Mycobacterium sp. TA27 with a Real-time PCR Product Detection System
- Transformation of Pseudomonas putida by Electroporation
- Survival and Impact of Genetically Engineered Pseudomonas putida Harboring Mercury Resistance Gene in Soil Microcosms
- Survival and Impact of Genetically Engineered Pseudomonas putida Harboring Mercury Resistance Gene in Aquatic Microcosms
- Linking Temporal Changes in Bacterial Community Structures with the Detection and Phylogenetic Analysis of Neutral Metalloprotease Genes in the Sediments of a Hypereutrophic Lake