Degradation Pathways of Trichloroethylene and 1,1,1-Trichloroethane by Mycobacterium sp. TA27(Microbiology & Fermentation Technology)

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概要

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• We analyzed the kinetics and metabolic pathways of trichloroethylene and 1,1,1-trichloroethane degradation by the ethane-utilizing Mycobacterium sp. TA27. The apparent V_<max> and K_m of trichloroethylene were 9.8 nmol min^<-1> mg of cells^<-1> and 61.9 μM, respectively. The apparent V_<max> and K_m of 1,1,1-trichloroethane were 0.11 nmol min^<-1> mg of cells^<-1> and 3.1 μM, respectively. 2,2,2-trichloroethanol, trichloroacetic acid, chloral, and dichloroacetic acid were detected as metabolites of trichloroethylene. 2,2,2-trichloroethanol, trichloroacetic acid, and dichloroacetic acid were also detected as metabolites of 1,1,1-trichloroethane. The amounts of 2,2,2-trichloroethanol, trichloroacetic acid, chloral, and dichloroacetic acid derived from the degradation of 3.60 μmol trichloroethylene were 0.16 μmol (4.4%), 0.11 μmol (3.1%), 0.02 μmol (0.6%), and 0.02 μmol (0.6%), respectively. The amounts of 2,2,2-trichloroethanol, trichloroacetic acid and dichloroacetic acid derived from the degradation of 1.73 μmol 1,1,1-trichloroethane were 1.48 μmol (85.5%), 0.22 μmol (12.7%), and 0.02 μmol (1.2%), respectively. More than 90% of theoretical total chloride was released in trichloroethylene degradation. Chloral and 2,2,2-trichloroethanol were transformed into each other, and were finally converted to trichloroacetic acid, and dichloroacetic acid. Trichloroacetic acid and dichloroacetic acid were not degraded by strain TA27.

• 社団法人日本農芸化学会の論文
• 2002-02-23

著者

• Yagi Osami

  Water And Soil Environmental Studies

• IWASAKI KAZUHIRO

  National Institute for Environmental Studies

• Hashimoto Akiko

  National Institute For Environmental Studies And Crest Japan Science And Technology Corporation

• Iwasaki K

  Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat

• Yagi O

  Univ. Tokyo Tokyo Jpn

• Yagi Osami

  Research Center For Water Environment Technology Graduate School Of Engineering The University Of To

• NAKASUGI Naou

  National Institute for Environmental Studies and CREST, Japan Science and Technology Corporation

• NAKAJIMA Mutsuyasu

  College of Bioresource Sciences, Nihon University

• Iwasaki K

  National Inst. For Environmental Studies Ibaraki Jpn

• Nakajima M

  College Of Bioresource Sciences Nihon University

• Nakasugi Naou

  National Institute For Environmental Studies And Crest Japan Science And Technology Corporation

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