Random Amplified Polymorphic DNA (RAPD) Analyses for Discriminating Genotypes of Microcystis Cyanobacteria
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概要
- 論文の詳細を見る
Random amplified polymorphic DNA (RAPD) analysis was used to discriminate genotypes in five species of Microcystis cyanobacteria. Strains of each group with the identical allozyme genotype (T. Kato et al., Algol, Stud., 1991, 129-140; M. Watanabe, in "Toxic Microcystis," ed. by M. F. Watanabe et al., CRC Press, Tokyo, 1966, pp. 13-34) gave similar RAPD patterns characterizing the respective group. On the other hand, no similarities in RAPD patterns were observed among strains of which allozyme genotypes were different. A good accordance between the RAPD analysis and allozyme divergence indicated a high reliability of both methods for discrimination of the affiliated groups of Microcystis. Several amplitied DNA fragment, which were expected to be markers for a particular taxon with identical allozyme genotype, were also observed on the RAPD patterns. Genetic homogeneities of M. novacekii, M. viridis, and M. wesenbergii were shown by RAPD analysis as well as the allozyme genotype. However, significant variations were observed in M. aeruginosa and M. ichthyoblabe in the levels of DNA and proteins (allozymes)
- 社団法人日本農芸化学会の論文
- 1997-07-23
著者
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WATANABE Masayuki
Department of Pediatrics and Developmental Medicine, Gunma University Graduate School of Medicine
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Yagi Osami
Water and Soil Environment Division, National Institute for Environmental Studies
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NISHIHARA HIROFUMI
Department of Agricultural Chemistry, The University of Tokyo
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Nishihara Hirofumi
Department Of Applied Biological Resource Science Ibaraki University
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Watanabe Masayuki
Department Of Botany National Science Museum
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Yagi Osami
Water And Soil Environmental Studies
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TAKAMURA YOSHICHIKA
Department of Bioresource Science, Faculty of Agriculture, Ibaraki University
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MIWA Hiroyasu
Department of Applied Biological Resource Sciences, Ibaraki University
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NAGASHIMA Minoru
Tsukuba Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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Miwa Hiroyasu
Department Of Applied Biological Resource Sciences Ibaraki University
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Nishihara H
Department Of Bioresource Sciences School Of Agriculture Ibaraki University
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Yagi O
Univ. Tokyo Tokyo Jpn
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Takamura Y
Department Of Bioresources Sciences School Of Agriculture Ibaraki University
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Takamura Yoshichika
Department Of Agricultural Chemistry Faculty Of Agriculture Ibaraki University
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Nagashima M
Tsukuba Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Watanabe Masayuki
Department Of Biology Graduate School Of Natural Science Konan University
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