Changes in Size of Intracellular Pools of Coenzyme A and Its Thioesters in Escherichia coli K-12 Cells to Various Carbon Sources and Stresses
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概要
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Intracellular pools of three CoA molecular species of coenzyme A, CoASH, acetyl-CoA, and malonyl-CoA, in Escherichia coli K-12 cells were studied by acyl-CoA cycling method in replacement culture. The sizes and compositions of CoA pools starved for a carbon source changed within minutes after the addition of one of various carbon sources. A large acetyl-CoA pool formed after the addition of D-glucose, D-fructose, D-mannose, glycerol. or sorbitol, but there was little change when L-glucose, sucrose, maltose, succinate, or acetate was added. The β-anomer of Dglucose was assimilated 10 times faster than the α-anomer. Intracellular CoA pools also changed with stress : in the pH, incubation temperature, or with osmotic stress. The sizes and compositions of CoA pools were not affected by pH changing between 4 and 8,but the breakdown of acetyl-CoA and CoASH was greater at pH 9 than at pH 4 to 8. Production of acetyl-CoA was greatest at 40℃, and at 50℃, and acetyl-CoA pool did not form at all and the size of the CoASH pool declined. When the organism was stressed by the addition of NaCl at concentrations of more than 0.6M, little acetyl-CoA was produced. The total CoA pool (the sum of the concentrations of CoASH, acetyl-CoA, and malonyl-CoA) remained within the limits of 0.83-1.40 nmol/mg of dry cell weight (0.30-0.52 mM). Whenever acetyl-CoA increased, CoASH decreased. Therefore, the acetyl-CoA/CoASH ratio is an important index of facultative anaerobes that reflects the state of carbon and energy metabolism in vivo.
- 社団法人日本農芸化学会の論文
- 1998-06-23
著者
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NISHIHARA HIROFUMI
Department of Agricultural Chemistry, The University of Tokyo
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Nishihara Hirofumi
Department Of Bioresource Sciences School Of Agriculture Ibaraki University
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Chohnan Shigeru
Department Of Agricultural Chemistry Faculty Of Agriculture Ibaraki University
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Chohnan Shigeru
Department Of Bioresource Sciences School Of Agriculture Ibaraki University
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TAKAMURA YOSHICHIKA
Department of Bioresource Science, Faculty of Agriculture, Ibaraki University
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IZAWA Hiroaki
Department of Bioresource Sciences, School of Agriculture, Ibaraki University
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Takamura Yoshichika
Department Of Agricultural Chemistry Faculty Of Agriculture Ibaraki University
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Takamura Yoshichika
Department Of Bioresource Sciences School Of Agriculture Ibaraki University
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Izawa Hiroaki
Department Of Bioresource Sciences School Of Agriculture Ibaraki University:(present Address)researc
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