Analysis of the Molecular Species of Hydrogenase in the Cells of an Obligately Chemolithoautotrophic, Marine Hydrogen-Oxidizing Bacterium, Hydrogenovibrio marinus(Microbiology & Fermentation Technology)
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概要
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Hydrogenovibrio marinus was suggested to have only membrane-bound hydrogenase (MBH). The change of cultivation pO_2 did not affect the molecular species of hydrogenase expressed. We propose the MBH is grouped in class I [NiFe] MBH according to the subunit composition, size (Mw 38,000 and Mw 74,000 subunits) and N-terminal sequences of the subunits, and arrangement of the structural genes. Ni-requirement for the autotrophic growth on H_2 also suggested the MBH is the Ni-containing type. Southern hybridization analysis using a part of the MBH gene showed a possibility of the presence of two highly homologous MBHs which were not separated by SDS-PAGE.
- 社団法人日本農芸化学会の論文
- 2001-12-23
著者
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NISHIHARA HIROFUMI
Department of Agricultural Chemistry, The University of Tokyo
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Miyata Yoshinori
Department Of Bioresource Science College Of Agriculture Ibaraki University
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Nishihara Hirofumi
Department Of Bioresource Science College Of Agriculture Ibaraki University
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Takamura Yoshichika
Department Of Agricultural Chemistry Faculty Of Agriculture Ibaraki University
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Takamura Yoshichika
Department Of Bioresource Science College Of Agriculture Ibaraki University
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MIYASHITA Youji
Department of Bioresource Science, College of Agriculture, Ibaraki University
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BERNHARD Michael
Institut fur Biologie, Humboldt Universitat zu Berlin
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POHLMANN Anne
Institut fur Biologie, Humboldt Universitat zu Berlin
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FRIEDRICH Barbel
Institut fur Biologie, Humboldt Universitat zu Berlin
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Pohlmann Anne
Institut Fur Biologie Humboldt Universitat Zu Berlin
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Friedrich Barbel
Institut Fur Biologie Humboldt Universitat Zu Berlin
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Bernhard Michael
Institut Fur Biologie Humboldt Universitat Zu Berlin
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