Functions of Malonate Decarboxylase Subunits from Pseudomonas putida(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
Malonate decarboxylase from Pseudomonas putida is composed of five subunits, α, β, γ, δ, and ε. Two subunits, δ and ε, have been identified as an acyl-carrier protein (ACP) and malonyl-CoA : ACP transacylase, respectively. Functions of the other three subunits have not been identified, because recombinant subunits expressed in Escherichia coli formed inclusion bodies. To resolve this problem, we used a coexpression system with GroEL/ES from E. coli, and obtained active recombinant subunits. Enzymatic analysis of the purified recombinant subunits showed that the α subunit was an acetyl-S-ACP : malonate ACP transferase and that the βγ-subunit complex was a malonyl-S-ACP decarboxylase.
- 社団法人日本農芸化学会の論文
- 2003-01-23
著者
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Chohnan Shigeru
Department Of Agricultural Chemistry Faculty Of Agriculture Ibaraki University
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TAKAMURA YOSHICHIKA
Department of Bioresource Science, Faculty of Agriculture, Ibaraki University
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AKAGI Kayo
Department of Bioresource Science, College of Agriculture, Ibaraki University
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Takamura Yoshichika
Department Of Agricultural Chemistry Faculty Of Agriculture Ibaraki University
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Akagi Kayo
Department Of Bioresource Science College Of Agriculture Ibaraki University
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