Quantitative and Specific Detection of a Trichloroethylene-degrading Methanotroph, Methylocystis sp. Strain M, by a Most Probable Number-Polymerase Chain Reaction Method(Microbiology & Fermentation Technology)
スポンサーリンク
概要
- 論文の詳細を見る
We developed a rapid and specific enumeration method for a trichloroethylene-degrading methanotroph, Methylocystis sp. strain M, based on a most probable number-polymerase chain reaction method for monitoring the bacterium at bioremediation sites. The primers designed for the mmoC gene of the soluble methane monooxygenase gene cluster were specific to strain M. Recovery of the cells with a membrane filter enabled us to detect strain M in trichloroethylene-contaminated groundwater. We used the enumeration method to monitor the number of strain M cells in effluent from soil columns supplied with trichloroethylene-contaminated groundwater. The number of strain M cells in the effluent depended on the amount of the strain M inoculated and the number of cells measured by the most probable numberpolymerase chain reaction method was correlated with that measured by a culture method. The detection limit for strain M in effluent detected by MPN-PCR method was 4 to 8×10^2 cells/ml.
- 社団法人日本農芸化学会の論文
- 2001-12-23
著者
-
NISHIHARA HIROFUMI
Department of Agricultural Chemistry, The University of Tokyo
-
Nishihara Hirofumi
Department Of Applied Biological Resource Science Ibaraki University
-
Yagi Osami
Water And Soil Environmental Studies
-
KIKUCHI Takeshi
Department of Materials Science, Kurashiki University of Science and the Arts
-
Nishihara H
Department Of Bioresource Sciences School Of Agriculture Ibaraki University
-
Iwasaki K
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat
-
Yagi O
Univ. Tokyo Tokyo Jpn
-
Yagi Osami
Research Center For Water Environment Technology Graduate School Of Engineering The University Of To
-
Takamura Y
Department Of Bioresources Sciences School Of Agriculture Ibaraki University
-
Takamura Yoshichika
Department Of Agricultural Chemistry Faculty Of Agriculture Ibaraki University
-
IWASAKI Kazuhiro
Biotechnology Risk Assessment Team, Biodiversity Conservation Research Project, National Institute f
-
Iwasaki K
National Inst. For Environmental Studies Ibaraki Jpn
-
Kikuchi Takeshi
Department Of Bioresource Sciences School Of Agriculture Ibaraki University
関連論文
- Occurrence of Hydrogen-Oxidizing Ralstonia Species as Primary Microorganisms in the Mt. Pinatubo Volcanic Mudflow Deposits(Soil Biology)
- Recovery of ^Cs by a Bioaccumulation System Using Rhodococcus erythropolis CS98
- Kinetic Studies on Cesium Transport in Rhodococcus erythropolis CS98 and Rhodococcus sp. Strain CS402
- Trichloroethylene Degradation by Cells of a Methane-Utilizing Bacterium, Methylocystis sp. M, Immobilized in Calcium Alginate
- On the Interaction of Ribosomal Protein L5 with 5S rRNA(Biochemistry & Molecular Biology)
- Isolation and Cultivation of Thermophilic Cyanobacteria from Hot Springs of Northern Thailand
- Production and Properties of Glycogen in the Marine Obligate Chemolithoautotroph, Hydrogenovibrio marinus
- Growth Characteristisc and High Cell-Density Cultivation of a Marine Obligately Chemolithoautotrophic Hydrogen-Oxidizing Bacterium Hydrogenovibrio marinus Strain MH-110 under a Continuous Gas-Flow System
- Requirement for C-terminal Extension to the RNA Binding Domain for Efficient RNA Binding by Ribosomal Protein L2(Biochemistry & Molecular Biology)
- Purification and Properties of a Soluble Methane Monooxygenase from Methylocystis sp. M
- Novel Metabolite of Trichloroethylene in a Methanotrophic Bacterium, Methylocystis sp. M, and Hypothetical Degradation Pathway
- Different Properties of Gene Products of Three Sets Ribulose 1, 5-Bisphosphate Carboxylase/Oxygenase from a Marine Obligately Autotrophic Hydrogen-Oxidizing Bacterium, Hydrogenovibrio marinus Strain MH-110
- Protein Phosphatase Inhivition Assay for Detection of Microcystins in Lake Water and Microcystis Cultures
- Random Amplified Polymorphic DNA (RAPD) Analyses for Discriminating Genotypes of Microcystis Cyanobacteria
- Growth Inhibition of Microcystis Cyanobacteria by L-Lysine and Disappearance of Natural Microcystis Blooms with Spraying
- Aerobic Degradation of Trichloroethylene by a New Type II Methane-Utilizing Bacterium, Strain M(Microbiology & Fermentation Industry)
- Aerobic Degradation of Trichloroethylene at High Concentration by a Methane-Utilizing Mixed Culture(Microbiology & Fermentation Industry)
- Nucleotide-Binding Sites in V-Type Na^+-ATPase from Enterococcus hirae
- Development of a Most-probable-number Method for Enumerating Denitrifying Bacteria by Using 96-Well Microtiter Plates and an Anaerobic Culture System
- Development of a New Most-probable-number Method for Enumerating Methanotrophs, Using 48-well Microtiter Plates
- Characteristics of Escherichia coli HB101 and Pseudomonas putida PpY101 Harboring a Recombinant Plasmid with Tandem Insertion of the Mercury Resistance Operon
- Immobilization of Trichloroethylene-Degrading Bacterium, Methylocystis sp. Strain M in Different Matrices
- Hepatocellular carcinoma with obstructive jaundice successfully treated with a self-expandable metallic stent
- Isolation and Characterization of the Slime from a Cyanobacterium, Microcystis aeruginosa K-3A(Microbiology & Fermentation Industry)
- A Simple Micromethod for Measurement of CoASH and Its Use in Measuring Intracellular Levels of CoASH and Short Chain Acyl-CoAs in Eschevichia coli K12 Cells(Microbiology & Fermentation Industry)
- Changes in Size of Intracellular Pools of Coenzyme A and Its Thioesters in Escherichia coli K-12 Cells to Various Carbon Sources and Stresses
- Functions of Malonate Decarboxylase Subunits from Pseudomonas putida(Biochemistry & Molecular Biology)
- Long-term Stability of Guided Tissue Regeneration Therapy in Intrabony Defects : A Case-control Study
- Degradation Pathways of Trichloroethylene and 1,1,1-Trichloroethane by Mycobacterium sp. TA27(Microbiology & Fermentation Technology)
- Quantitative and Specific Detection of a Trichloroethylene-degrading Methanotroph, Methylocystis sp. Strain M, by a Most Probable Number-Polymerase Chain Reaction Method(Microbiology & Fermentation Technology)
- Quantitative Detection of Trichloroethylene-Degrading Mycobacterium sp. TA27 with a Real-time PCR Product Detection System
- Transformation of Pseudomonas putida by Electroporation
- Survival and Impact of Genetically Engineered Pseudomonas putida Harboring Mercury Resistance Gene in Soil Microcosms
- Survival and Impact of Genetically Engineered Pseudomonas putida Harboring Mercury Resistance Gene in Aquatic Microcosms
- Malonate Decarboxylase in Bacteria and Its Application for Determination of Intracellular Acyl-CoA Thioesters
- Analysis of the Molecular Species of Hydrogenase in the Cells of an Obligately Chemolithoautotrophic, Marine Hydrogen-Oxidizing Bacterium, Hydrogenovibrio marinus(Microbiology & Fermentation Technology)
- Synthesis and properties of the system Al_2(SO_4)_3-Fe_2(SO_4)_3
- A case of mixed connective tissue disease presenting with nephrotic syndrome
- Measurement of Intracellular Acetyl-CoA and Malonyl-CoA in Bacteria and Yeasts Using Acyl-CoA Cycling(Microbiology & Fermentation Industry)
- Differentiation of Thermophilic Anaerobic Gram-positive Bacteria by Random Amplified Polymorphic DNA Analysis
- Analytic calculation of cation distribution equilibria in the cubic phase of jacobsite-hausmannite system
- Local Skin Flap Reconstruction for Abdominal Wound Dehiscence After Abdominal Surgery with a Stoma : Report of Two Cases
- Curvature entropy trapping of long DNA under hydrodynamic flows in microfluidic devices
- Concentration and injection of long deoxyribonucleic acid molecules at the interface of micro- and nano-channels
- Vertebrobasilar Infarction Related to Giant Cell (Temporal) Arteritis: Case Report
- CHEMICAL INVESTIGATION OF THE SKIPPER OIL