Growth Characteristisc and High Cell-Density Cultivation of a Marine Obligately Chemolithoautotrophic Hydrogen-Oxidizing Bacterium Hydrogenovibrio marinus Strain MH-110 under a Continuous Gas-Flow System
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概要
- 論文の詳細を見る
A marine obligately chemolithoautotrophic aerobic hydrogen-oxidizing bacterium Hydrogenovibrio marinus strain MH-110 was cultivated in a 2-l jar fermentor with continuous supply of a mixed gas of H_2/O_2 /CO_2. Fast growth began rapidly without a lag period, even in an atmosphere of 40% oxygen. Apparent specific growth rate of the initial stage of growth was 0.60-0.67 h^<-1> irrespective of any oxygen tension tested. Under an atmosphere containing hydrogen, the growth was promoted by the addition of tetrathionate to the medium. A dense culture was achieved by increasing the tension of oxygen and adding ferrous and magnesium ions. before these factors became growth limiting. Under this culture condition, exponential growth contiuned until the cell density reached 14 g/l dry weight. THe final cell concentration reached approximately 26 g/l dry weight after 30-h cultivation.
- 社団法人日本生物工学会の論文
- 1991-11-25
著者
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IGARASHI Yasuo
Department of Biotechnology, The University of Tokyo
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Igarashi Y
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Igarashi Yasuo
University Of Tokyo
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KODAMA Tohru
Department of Textile Science and Technology, Shinshu University
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Igarashi Yasuo
Department Of Biotechnology The University Of Tokyo
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Igarashi Y
Univ. Tokyo Tokyo Jpn
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NISHIHARA HIROFUMI
Frontier Technology Research Institute, Tokyo Gac Co. Ltd.
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Nishihara Hirofumi
Department Of Applied Biological Resource Science Ibaraki University
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Igarashi Yasuo
Department Of Agricultural Chemistry The University Of Tokyo
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Kokubo Tetsuro
Department Of Biotechnology The University Of Tokyo:(present Address)division Of Gene Function In An
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Nishihara H
Department Of Bioresource Sciences School Of Agriculture Ibaraki University
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Kodama Tohru
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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