Culture-Independent Phylogenetic Analysis of the Microbial Community in Industrial Sugarcane Bagasse Feedstock Piles

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概要

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• Sugarcane bagasse is an important lignocellulosic by-product with potential for conversion to biofuels and chemicals in biorefinery. As a step towards an understanding of microbial diversity and the processes existing in bagasse collection sites, the microbial community in industrial bagasse feedstock piles was investigated. Molecular biodiversity analysis of 16S rDNA sequences revealed the presence of a complex bacterial community. A diverse group of mainly aerobic and facultative anaerobic bacteria was identified reflecting the aerobic and high temperature microenvironmental conditions under the pile surface. The major bacterial taxa present were identified as Firmicutes, Alpha- and Gammaproteobacteria, Acidobacteria, Bacteroidetes, and Actinobacteria. Analysis of the eukaryotic microbial assemblage based on an internal transcribed spacer revealed the predominance of diverse cellulolytic and hemicellulolytic ascomycota. A microbial interaction model is proposed, focusing on lignocellulose degradation and methane metabolism. The insights into the microbial community in this study provide a basis for efficient utilization of bagasse in lignocellulosic biomass-based industries.

• 社団法人 日本農芸化学会の論文
• 2011-02-23

著者

• KANOKRATANA Pattanop

  Enzyme Technology Laboratory, National Center for Genetic Engineering and Biotechnology

• Igarashi Yasuo

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Igarashi Yasuo

  Department Of Agricultural Chemistry The University Of Tokyo

• Champreda Verawat

  Enzyme Technology Laboratory National Center For Genetic Engineering And Biotechnology

• Eurwilaichitr Lily

  Enzyme Technology Laboratory National Center For Genetic Engineering And Biotechnology

• Rattanachomsri Ukrit

  Enzyme Technology Laboratory National Center For Genetic Engineering And Biotechnology

• Kanokratana Pattanop

  Enzyme Technology Laboratory National Center For Genetic Engineering And Biotechnology

• Champreda Verawat

  Enzyme Technology Laboratory Bioresources Technology Unit National Center For Genetic Engineering And Biotechnology

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