Cloning and Characterization of the Genes Involved in the Degradation of Dibenzofuran by Terrabacter sp. Strain DBF63

スポンサーリンク

概要

• 論文の詳細を見る

• Strain DBF63 utilizes dibenzofuran (DBF) as the sole source of carbon and energy. This strain was identified as a Terrabacter sp. on the basis of the phylogenetic analysis of the sequence of the 16s rRNA gene, in addition to its morphological, biochemical and chemical properties. Two genes encoding extradiol dioxygenases were cloned from strain DBF63 by shot-gun cloning on the basis of the expressed activity of extradiol dioxygenases for 2,3-dihydroxybiphenyl (DHB). One of two clones, E. coli MV1184 (pLM1), exhibited, a higher level of activity for DHB than for 3-methylcatechol (3MC), whlie the other, MV1184 (pKN1) exhibited a low level of activity for both catechol compounds. In addition, the gene carried on pLM1 was lost in strain DBF63W, which is a mutant of strain DBF63 deficient in the ability to utilize DBF and obtained as a result of cintnuous culture on nutrient broth. These results suggest that the gene (dbfB) carried on pLM1 encodes the extradiol dioxygenase involved in the degradation of DBF in strain DBF63. Nucleotide sequencing of the 8.6-kb KpnI-StuI DNA fragment including dbfB revealed the existence of a hydrolase gene (dbfC), a gene encoding a phenol hydroxylase-like polypeptide, and a region homologous to IS401 of Pseudomonas cepacia ATCC17616. Co-expression in E. coli of dbfBC and carA, the gene products of which catalyze the initial oxidation of DBF to 2,2', 3-trihydroxybiphenyl (THB), revealed that the products of dbfBC were capable of degrading THB to salicylate. It was also shown that E. coli carrying both carA and dbfBC was capable of degrading dibenzo-p-dioxin (DD).

• 社団法人日本生物工学会の論文
• 1997-11-25

著者

• KODAMA Tohru

  Department of Textile Science and Technology, Shinshu University

• NOJIRI Hideaki

  Biotechnology Research Center, The University of Tokyo

• YAMANE Hisakazu

  Biotechnology Research Center, The University of Tokyo

• OMORI TOSHIO

  Biotechnology Research Center, The University of Tokyo

• Omori T

  Coll. Of Systems Engineering And Sci. Dep. Of Bioscience And Engineering Shibaura Inst. Of Technol.

• KASUGA KANO

  Biotechnology Research Center

• Omori Toshio

  Biotechnoiogy Researchcenter The University Of Tokyo

• Nojiri H

  Biotechnology Res. Center The Univ. Of Tokyo

• Noguchi Haruko

  Biotechnology Research Center The University Of Tokyo

• Yamane Hisakazu

  Biotechnology Res. Center The Univ. Of Tokyo

• Yamane Hisakazu

  Biotechnology Res. Center Univ. Of Tokyo

• Yamane Hisakazu

  Biotech. Res. Ctr. The Univ. Of Tokyo

• Nojiri Hideaki

  Biotechnology Res. Center The Univ. Of Tokyo

• Kasuga Kano

  Biotechnology Research Center:the University Of Tokyo

• Kodama T

  Department Of Biotechnology The University Of Tokyo:(present Address)faculty Of Textile Science And

• Kodama Tohru

  Department Of Agricultural Chemistry The University Of Tokyo

• Kokubo Tetsuro

  Department Of Biotechnology The University Of Tokyo:(present Address)division Of Gene Function In An

• Kodama Tohru

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Yada H

  Biotechnology Research Center University Of Tokyo

• Omori Toshio

  Shibaura Institute Of Technology

• YAMANE Hisakazu

  Biotech. Res. Ctr. , The Univ. of Tokyo

関連論文

• Carboxylation Reactions of Pyruvate:Ferredoxin Oxidoreductase and 2-Oxoglutarate:Ferredoxin Oxidoreductase from Hydrogenobacter thermophilus TK-6
• Purification and Characterization of Ferredoxin from Hydrogenobacter thermophilus Strain TK-6
• Purification and Some Properties of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase from a Thermophilic Hydrogen-Oxidizing Bacterium, Pseudomonas hydrogeneothermophila Strain TH-1
• Methionaquinone is a Direct Natural Electron Acceptor for the Membrane-bound Hydrogenase in Hydrogenobacter thermophilus Strain TK-6(Biological Chemistry)
• Colony Formation of Hydrogenobacter thermophilus on a Plate Solidified with GELRITE(Microbiology & Fermentation Industry)
• Purification and Some Properties of Cytochrome c552 from Hydrogenobacter thermophilus
• The Membrane-bound Hydrogenase Reduces Cytochrome c552 in Hydrogenobacter thermophilus strain TK-6
• A Series of Crystal Structures of a meta-Cleavage Product Hydrolase from Pseudomonas fluorescens IP01 (CumD) Complexed with Various Cleavage Products
• Purification, Characterization, and Steady-State Kinetics of a meta-Cleavage Compound Hydrolase from Pseudomonas fluorescens IP01
• Expression Pattern of the CsPK3 Auxin-Responsive Protein Kinase Gene
• Detection of a Bacterial Group within the Phylum Chloroflexi and Reductive-Dehalogenase-Homologous Genes in Pentachlorobenzene-Dechlorinating Estuarine Sediment from the Arakawa River, Japan
• Genes Involved in Aniline Degradation by Delftia acidovorans Strain 7N and Its Distribution in the Natural Environment
• Isolation and Characterization of an Alkaliphilic Bacterium Utilizing Pyrene as a Carbon Source(ENVIRONMENTAL BIOTECHNOLOGY)
• Divergent Structures of Carbazole Degradative car Operons Isolated from Gram-negative Bacteria
• Rhizoremediation of Dioxin-like Compounds by a Recombinant Rhizobium tropici Strain Expressing Carbazole 1,9a-Dioxygenase Constitutively(Microbiology & Fermentation Technology)
• Expression, Purification, and Characterization of 2'-Aminobiphenyl-2,3-diol 1,2-dioxygenase from Carbazole-degrader Pseudomonas resinovorans Strain CA10(Microbiology & Fermentation Technology)
• Purification and Characterization of meta-Cleavage Compound Hydrolase from a Carbazole Degrader Pseudomonas resinovorans Strain CA10(Microbiology & Fermentation Technology)
• Cloning and Characterization of cDNAs for the Jasmonic Acid-responsive Genes RRJ1 and RRJ2 in Suspension-cultured Rice Cells(Biochemistry & Molecular Biology)
• Organization and Transcriptional Characterization of Catechol Degradation Genes Involved in Carbazole Degradation by Pseudomonas resinovorans Strain CA10(Microbiology & Fermentation Technology)
• Evidence for the Presence of DNA-Binding Proteins Involved in Regulation of the Gene Expression of Indole-3-Pyruvic Acid Decarboxylase, a Key Enzyme in Indole-3-Acetic Acid Biosynthesis in Azospirillum lipoferum FS
• New Classification System for Oxygenase Components Involved in Ring-Hydroxylating Oxygenations
• Isolation and Characterization of Low-indole-3-acetic Acid-producing Mutants from Bradyrhizobium elkanii
• Polypeptide Requirement of Multicomponent Monooxygenase DsoABCDEF for Dimethyl Sulfide Oxidizing Activity
• Cloning and Characterization of the Genes Involved in the Degradation of Dibenzofuran by Terrabacter sp. Strain DBF63
• Cloning and Nucleotide Sequences of the Genes Involved in the meta-Cleavage Pathywa of Cumene Degradation in Pseudomonas fluorescens IP01
• Cloning, Nucleotide Sequence, and Characterization of the Genes Encoding Enzymes Involved in the Degradation of Cumene to 2-Hydroxy-6-Oxo-7-Methylocta-2,4-Dienoic Acid in Pseudomonas fluorescens IP01
• Desulfurization of Alkyl and Aromatic Sulfides and Sulfonates by Dibenzothiophene-desulfurizing Rhodococcus sp. Strain SY1
• Diterpene Phytoalexins Are Biosynthesized in and Exuded from the Roots of Rice Seedlings
• Involvement of the Basic Helix-Loop-Helix Transcription Factor RERJ1 in Wounding and Drought Stress Responses in Rice Plants
• Preparation and Biological Activity of Molecular Probes to Identify and Analyze Jasmonic Acid-binding Proteins
• 1P-023 芳香環水酸化ジオキシゲナーゼの電子伝達コンポーネント(ferredoxin reductase)のX線結晶構造解析(蛋白質・構造(1),第46回日本生物物理学会年会)
• CLONING OF ent-KAURENE SYNTHASE B cDNA FROM PUMPKIN(Cucurbita maxima L. )
• Elicitor and Calatalse Activity of Conidia Suspensions of Various Strains of Magnaporthe grisea in Suspension-Cultured Cells of Rice
• Induction of Resistance against Rice Blast Fungus in Rice Plants Treated with a Potent Elicitor, N-Acetylchitooligosaccharide
• Characterization of an Elicitor-Induced Rice WRKY Gene, OsWRKY71
• Pael receptor is involved in dopamine metabolism in the nigrostriatal system
• Purification and Characterization of Membrane-bound Hydrogenase from a Thermophilic Hydrogen-Oxidizing Bacterium, Pseudomonas hydrogenothermophila Strain TH-1
• Isolation and Cultivation of Thermophilic Cyanobacteria from Hot Springs of Northern Thailand
• Electron Transport System in Pseudomonas hydrogenothermophila Strain TH-1, a Thermophilic Hydrogen-oxidizing Bacterium
• Diterpene Cyclases Responsible for the Biosynthesis of Phytoalexins, Momilactones A, B, and Oryzalexins A-F in Rice
• Isolation and Identification of Antheridiogens in the Ferns, Lygodium microphyllum and Lygodium reticulatum(Organic Chemistry)
• Cloning and Sequence of the L_2 Form of RubisCO from a Marine Obligately Autotrophic Hydrogen-oxidizing Bacterium, Hydrogenovibrio marinus Strain MH-110
• Production and Properties of Glycogen in the Marine Obligate Chemolithoautotroph, Hydrogenovibrio marinus
• Growth Characteristisc and High Cell-Density Cultivation of a Marine Obligately Chemolithoautotrophic Hydrogen-Oxidizing Bacterium Hydrogenovibrio marinus Strain MH-110 under a Continuous Gas-Flow System
• Thermostability of Pseudomonas hydrogenothermophila Cytochrome c-552
• Bacterial Lipopolysaccharides Induce Defense Responses Associated with Programmed Cell Death in Rice Cells
• Production of Recombinant Cytochrome c-551 in a Pseudomonas aeruginosa Mutant Strain
• Structure and ANR-Dependent Transcription of the nir Genes for Denitritication from Pseudomonas aeruginosa
• Heterologous Expression of Hydrogenobacter thermophilus Cytochrome c-552 in the Periplasm of Pseudomonas aeruginosa
• cDNA CLONING OF ent-KAURENE SYNTHETASEA FROM RICE
• Identification of Gibberellins and 9,15-Cyclogibberellins in Developing Apple Seeds
• Gibberellink and Antheridiogens in Prothallia and Sporophytes of Anemia phyllitidis
• Effects of Fluorogibberellins on Plant Growth and Gibberellin 3β-Hydroxylases
• Inhibition of ent-Kaurene Synthase by Quaternary Ammonium Growth Retardants
• Effects of Gibberellins and Prohexadione on the Activities of Oryzain and α-Amylase in Rice Seeds : PROTEINS, ENZYMES AND METABOLISM
• Promotive Effect of Auxins on UDP-Glucose : Flavonol Glucosyltransferase Activity in Vitis sp.Cell Cultures
• Quercetin 3-0-β-D-Glucopyranoside and Isorhamnetin 3-O-β-D-Glucopyranoside Formation from Quercetin by Cell Cultures of Ipomoea batatas and Crocus sativum(Biological Chemistry)
• Glucosylation of Quercetin by a Cell Suspension Culture of Vitis sp.(Biological Chemistry)
• Cell Mass Production of Acinetobacter calcoaceticus in Palm Oil-Limited Chemostant Culture
• Cloning, Sequence and Overexpression of the Thermophilic Cyanobacterium Gene for the Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase
• Purification of RuBisCO from the Thermophilic Cyanobacterium Synechococcus sp. Strain a-1
• Qualitative and Quantitative Analysis of Endogenous Gibberellins in Raphanus sativus L. during Cold Treatment and the Subsequent Growth
• Construction of Novel Expression Vectors Effective in Pseudomonas Cells(Microbiology & Fermentation Industry)
• Alteration of the Substrate Specificity of the Angular Dioxygenase Carbazole 1,9a-Dioxygenase
• Cloning and Expression of a Clostridium cellobioparum Cellulase Gene and Its Excretion from Escherichia coil JM109
• Acetoacetyl-CoA Transferase and Thiolase Reactions Are Catalyzed by a Single Enzyme in Clostridium acetobutylicum ATCC824(Microbiology & Fermentation Industry)
• Molecular Cloning and Sequence Analysis of the Gene Encoding an Endo _α-1,4 Polygalactosaminidase of Pseudomonas sp. 881
• Biodegradation of Carbazole by Pseudomonas spp. CA06 and CA10
• A Non-radioisotopic Anion-exchange Chromatographic Method to Measure the CO_2/O_2 Specificity Factor for Ribulose Bisphosphate Carboxylase/Oxygenase
• Effect of Carbon Cioxide Concentration on the Growth and RubisCO Activity of a Thermophilic Cyanobacterium, Chroococcidiopsis sp. Strain TS-821
• Preparation of (±)-2-(2,3-^2H_2)Jasmonic Acid and Its Methyl Ester, Methyl (±)-2-(2,3-^2H_2)Jasmonate
• Behavior of Various Hosts of the IncP-7 Carbazole-Degradative Plasmid pCAR1 in Artificial Microcosms
• The Behavior and Significance of Degradative Plasmids Belonging to Inc Groups in Pseudomonas within Natural Environments and Microcosms
• The Complete Nucleotide Sequence of pCAR2: pCAR2 and pCAR1 Were Structurally Identical IncP-7 Carbazole Degradative Plasmids
• Characterization of Chilli Pepper hairy Roots Expressing the Parsley PAL2 cDNA
• Transformation of Chilli Pepper (Capsicum frutescens) with a Phenylalanine Ammonia-Lyase Gene
• トウガラシ(Capsicum frutescens)の毛状根培養
• Mode of Action for Mitomycin C Inactivating Factors of Pseudomonas convexa 4-87(Biological Chemistry)
• Partial Purification and Some Properties of Mitomycin C Inactivating Factors of Pseudomonas convexa 4-87(Biological Chemistry)
• Genetic Evidence for the Role of Isopentenyl Diphosphate Isomerases in the Mevalonate Pathway and Plant Development in Arabidopsis
• Release of neurotransmitters in the monkey frontal cortex is related to level of attention
• Molecular Bases of Aerobic Bacterial Degradation of Dioxins : Involvement of Angular Dioxygenation
• Effects of Salicylic Acid on the Production of Procyanidin and Anthocyanin in Cultured Grape Cells
• Microbial Degradation of the Polycyclic Aromatic Hydrocarbons Acenaphthene and Acenaphthylene by a Pure Bacterial Culture
• Promoter Analysis of the Elicitor-Induced WRKY Gene OsWRKY53, Which Is Involved in Defense Responses in Rice
• Alteration of the Substrate Specificity of the Angular Dioxygenase Carbazole 1,9a-Dioxygenase
• Relative power contributions of unit discharges simultaneously recorded in the mesencephalic reticular formation
• Acetylcholine and glutamate release during sleep-wakefulness in the pedunculopontine tegmental nucleus and norepinephrine changes regulated by nitric oxide
• Characterization of an Elicitor-Induced Rice WRKY Gene, OsWRKY71
• Identification of the Electron Transfer Flavoprotein as an Upregulated Enzyme in the Benzoate Utilization of Desulfotignum balticum
• Identification of the Electron Transfer Flavoprotein as an Upregulated Enzyme in the Benzoate Utilization of Desulfotignum balticum
• The Behavior and Significance of Degradative Plasmids Belonging to Inc Groups in Pseudomonas within Natural Environments and Microcosms
• Behavior of Various Hosts of the IncP-7 Carbazole-Degradative Plasmid pCAR1 in Artificial Microcosms
• The Complete Nucleotide Sequence of pCAR2 : pCAR2 and pCAR1 Were Structurally Identical IncP-7 Carbazole Degradative Plasmids
• Identification of a Jasmonic Acid-Responsive Region in the Promoter of the Rice 12-Oxophytodienoic Acid Reductase 1 Gene OsOPR1
• Oligomerization and DNA-Binding Capacity of Pmr, a Histone-Like Protein H1 (H-NS) Family Protein Encoded on IncP-7 Carbazole-Degradative Plasmid pCAR1
• Functional Identification of a Rice ent-Kaurene Oxidase, OsKO2, Using the Pichia pastoris Expression System
• Suppressive effect of abscisic acid on systemic acquired resistance in tobacco plants
• Membrane Topology and Functional Analysis of Methylobacillus sp. 12S Genes epsF and epsG, Encoding Polysaccharide Chain-Length Determining Proteins
• OsJAR1 Contributes Mainly to Biosynthesis of the Stress-Induced Jasmonoyl-Isoleucine Involved in Defense Responses in Rice

もっと見る 閉じる

スポンサーリンク