Cloning, Sequence and Overexpression of the Thermophilic Cyanobacterium Gene for the Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase

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概要

• 論文の詳細を見る

• The genes for the large (rbcL) and small (rbcS) subunits of ribulose-1,5-bisphosphate chaboxylase/oxygenase (RubisCO) were cloned from a thermophilic cyanobacterium, Synechococcus sp. strain a-1. These two genes were located tandemly on the same strand to chromosomal DNA with a 467bp spacer region. The rbcL gene codes for 474 amino acid residues (1,422bp), and rbcS for 118 residues (354bp). The deduced amino acid sequence for the large subunit was highly similar to those of other cyanobacteria and higher plants. The RubisCO genes were overexpressed (>30% of total soluble protein) in E. coli cells by using pKK223-3 as an expression vector. The overexpressed RubisCO formed hexadecamers (L_8S_8form) in E. cili cells, the same form as in Synechococcus cells. The RubisCO was easily purified from E. coli cell free extract using the advantage of its high termostability, and the purified RubisCO had almost the same characteristics as the native RubisCO purified from Synechococcus sp. strain a-1.

• 社団法人日本生物工学会の論文
• 1993-01-25

著者

• IGARASHI Yasuo

  Department of Biotechnology, The University of Tokyo

• Igarashi Y

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Igarashi Yasuo

  University Of Tokyo

• KODAMA Tohru

  Department of Textile Science and Technology, Shinshu University

• Chung Seon

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research (riken)

• Chung S

  Department Of Biotechnology University Of Tokyo

• Igarashi Y

  Univ. Tokyo Tokyo Jpn

• Yaguchi Toshiaki

  Department of Biotechnology, The University of Tokyo

• Chung Seong

  Department of Biotechnology, The University of Tokyo

• Igarashi Yasuo

  Department Of Agricultural Chemistry The University Of Tokyo

• Kodama T

  Department Of Biotechnology The University Of Tokyo:(present Address)faculty Of Textile Science And

• Kokubo Tetsuro

  Department Of Biotechnology The University Of Tokyo:(present Address)division Of Gene Function In An

• Chung Seong

  Department Of Biotechnology University Of Tokyo

• YAGUCHI TOSHIAKI

  Institute for Fundamental Research, Suntory Limited

• Yaguchi Toshiaki

  Institute For Fundamental Research Suntory Limited

• Kodama Tohru

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

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