Promotive Effect of Auxins on UDP-Glucose : Flavonol Glucosyltransferase Activity in Vitis sp.Cell Cultures

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概要

• 論文の詳細を見る

• The addition of 2, 4-dichlorophenoxyacetic acid(2, 4-D)to Vitis sp.cell cultures significantly enhanced the production of quercetin 3, 7, 4′-tri-O-glucoside, 3, 7-di-O-glucoside and 3, 4′-di-O-glucoside from quercetin. This enhancement of glucosylation by 2, 4-D was also observed in cell cultures of other plant species. The activity of UDP-glucose: flavonol glucosyltransferase(UFGT)in cell-free extracts of Vitis sp.cell cultures increased approximately 10-fold, 48 h after the addition of 2, 4-D to the culture medium. The UFGT activity increased linearly up to 15 h and showed a maximal response to the addition of 10-50 mg/l of 2, 4-D at 48 h. The promotive effect of 2, 4-D was inhibited by cycloheximide suggesting that de novo protein synthesis was involved in this phenomenon. Interestingly, similar promotive effects on the UFGT activity were observed for other phytohormones such as kinetin and several anti-auxins.

• 社団法人日本生物工学会の論文
• 2001-06-25

著者

• YAMAKAWA Takashi

  Department of Global Agricultural Sciences, The University of Tokyo

• KODAMA Tohru

  Department of Textile Science and Technology, Shinshu University

• Nakamura Matsumi

  Rumen Microbiology Research Team Staff-institute

• Nakamura Mayumi

  Department Of Biotechnology The University Of Tokyo

• Yamakawa Takashi

  Laboratory Of Plant Biotechnology Department Of Global Agricultural Sciences The University Of Tokyo

• Yamakawa Takashi

  Department Of Biotechnology The University Of Tokyo:(present Address)department Of Global Agricultur

• Yamakawa Takashi

  Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of

• Yamakawa Takashi

  Biotechnology Research Center Toyama Prefectural University

• Yamakawa T

  Laboratory Of Plant Biotechnology Department Of Global Agricultural Sciences The University Of Tokyo

• KOKUBO TETSURO

  Department of Biotechnology, The University of Tokyo

• AMBE-ONO YUKIKO

  Department of Biotechnology, The University of Tokyo

• ISHIDA HIDEKATSU

  Department of Biotechnology, The University of Tokyo

• Ambe-ono Yukiko

  Department Of Biotechnology The University Of Tokyo

• Kodama T

  Department Of Biotechnology The University Of Tokyo:(present Address)faculty Of Textile Science And

• Kodama Tohru

  Department Of Agricultural Chemistry The University Of Tokyo

• Kokubo Tetsuro

  Department Of Biotechnology The University Of Tokyo:(present Address)division Of Gene Function In An

• Nakamura M

  Rumen Microbiology Research Team Staff-institute

• Kodama Tohru

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Ishida H

  Department Of Biotechnology The University Of Tokyo

• Nakamura Mayumi

  Department Of Biological Science Faculty Of Science Shizuoka University

• Yamakawa Takashi

  Department Of Agricultural Chemistry University Of Tokyo

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