Excretion of L-Tryptophan by Analogue-resistant Mutants of Pseudomonas hydrogenothermophila TH-1 in Autotrophic Cultures
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概要
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Cells of a thermophilic hydrogen bacterium, Pseudomonas hydrogenothermophila TH-1 were treated with N-methyl-N-nitro-TV-nitrosoguanidine and resulting mutants resistant to tryptophan analogues were selected under autotrophic culture conditions (energy source, H2; carbon source, CO2). A mutant strain, 7922, which was resistant to 2000 μg/ml of 5-methyltryptophan and 200 - 500 μg/ml of 5-fluorotryptophan, was obtained by two step mutations. This mutant excreted 38 - 70 μg/ml of tryptophan into flask culture broth and a maximum of 200 μg/ml into jar fermentor broth.
- 社団法人 日本農芸化学会の論文
著者
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Minoda Yasuji
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Igarashi Yasuo
Department Of Agricultural Chemistry The University Of Tokyo
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Kodama Tohru
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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