Purification and Some Properties of 2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid(HOPDA) Reducing Enzyme from Pseudomonas cruciviae S93B1 Involved in the Degradation of Biphenyl(Biological Chemistry)

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概要

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• Pseudomonas cruciviae S93B1 produced three HOPDA reducing enzymes (I, II, III), found by chromatography on DEAE-Sepharose CL-6B. The purified HOPDA reducing enzyme III was homogeneous on polyacrylamide gel and has a mol. wt. of about 170,000 by Sephadex G-200. The reducing enzyme III produced 2,6-dioxo-6-phenylhexanoic acid from HOPDA. NADPH was an essential cofactor for HOPDA reducing enzyme III. The enzyme was stable between pH 5 and 7. The ring-fission products from biphenyl-2,3-diol, 3-isopropylcatechol, 3-methylcatechol and the methyl ester of HOPDA were substrates for the reducing enzymes I, II and III. The ring-fission products from 3-methoxycatechol and catechol served as substrates for reducing enzymes I and II. The methyl ester of HOPDA was reduced by reducing enzyme III to 2-methoxy-6-oxo-6-phenylhexa-2-enoic acid methyl ester.

• 社団法人日本農芸化学会の論文
• 1986-06-23

著者

• OMORI Toshio

  Department of Industrial Chemistry, Shibaura Institute of Technology

• Omori T

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• MINODA Yasuji

  Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo

• ISHIGOOKA Hiroshi

  Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo

• Omori Toshio

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Minoda Y

  Univ. Tokyo Tokyo Jpn

• Minoda Yasuji

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Ishigooka Hiroshi

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

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