Purification and Some Properties of a 2-Hydroxy-6-oxo-6-phenylhexa-2, 4-dienoic Acid Hydrolyzing Enzyme from Pseudomonas cruciviae S93 Bl involved in the Degradation of Biphenyl
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概要
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A 2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoic acid (HOPDA) hydrolyzing enzyme was purified to a homogeneous state from P. cruciviae S93 Bl grown on biphenyl as the carbon source. The enzyme hydrolyzed HOPDA between the C5-C6 bond to produce benzoic acid and 2-oxopent-4-enoic acid. The HOPDA hydrolyzing enzyme had a molecular weight of about 160, 000, and was dissociated in SDS, with prior treatment with mercaptoethanol, into a single subunit with an approximate molecular weight of 29, 000. The optimum pH of this hydrolase was 4.7, and it was relatively stable in an alkaline solution. It attacked the meta ring-fission products of biphenyl-2, 3-diol, 3-isopropylcatechol, 3-methylcatechol and catechol. It was proposed that this enzyme is called 2, 6-dioxo-6-phenylhexa-3-enoic acid (DPEA) by hydrolase and is classified under the number EC 3.7.1.
- 社団法人 日本農芸化学会の論文
著者
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OMORI Toshio
Department of Industrial Chemistry, Shibaura Institute of Technology
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MINODA Yasuji
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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ISHIGOOKA Hiroshi
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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SUGIMURA Keijiro
Department of Agricultural Chemistry, Faculty of Agriculture, Tne University of Tokyo
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OMORI Toshio
Department of Agricultural Chemistry, Faculty of Agriculture, Tne University of Tokyo
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