Identification of the Electron Transfer Flavoprotein as an Upregulated Enzyme in the Benzoate Utilization of Desulfotignum balticum
スポンサーリンク
概要
- 論文の詳細を見る
Desulfotignum balticum utilizes benzoate coupled to sulfate reduction. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) analysis was conducted to detect proteins that increased more after growth on benzoate than on butyrate. A comparison of proteins on 2D gels showed that at least six proteins were expressed. The N-terminal sequences of three proteins exhibited significant identities with the α and β subunits of electron transfer flavoprotein (ETF) from anaerobic aromatic-degraders. By sequence analysis of the fosmid clone insert (37,590 bp) containing the genes encoding the ETF subunits, we identified three genes, whose deduced amino acid sequences showed 58%, 74%, and 62% identity with those of Gmet_2267 (Fe-S oxidoreductase), Gmet_2266 (ETF β subunit), and Gmet_2265 (ETF α subunit) respectively, which exist within the 300-kb genomic island of aromatic-degradation genes from Geobacter metallireducens GS-15. The genes encoding ETF subunits found in this study were upregulated in benzoate utilization.
- 社団法人 日本農芸化学会の論文
著者
-
NOJIRI Hideaki
Biotechnology Research Center, The University of Tokyo
-
YAMANE Hisakazu
Biotechnology Research Center, The University of Tokyo
-
OMORI Toshio
Department of Industrial Chemistry, Shibaura Institute of Technology
-
ENDOH Takayuki
Biotechnology Research Center, The University of Tokyo
-
WATANABE KAZUYA
Research Center for Advanced Science and Technology, The University of Tokyo
-
Habe Hiroshi
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industria
-
Hosoda Akifumi
Department Of Environmental Bioscience Meijo University
-
TAMURA Hiroto
Department of Applied Biological Chemistry, Meijo University
-
Habe H
Research Institute For Innovations In Sustainable Chemistry National Institute Of Advanced Industria
-
KOBUNA Akinori
Biotechnology Research Center, The University of Tokyo
-
KOSAKA Tomoyuki
PRESTO, Japan Science and Technology Agency (JST)
関連論文
- Disruption of the Membrane-Bound Alcohol Dehydrogenase-Encoding Gene Improved Glycerol Use and Dihydroxyacetone Productivity in Gluconobacter oxydans
- A Series of Crystal Structures of a meta-Cleavage Product Hydrolase from Pseudomonas fluorescens IP01 (CumD) Complexed with Various Cleavage Products
- Purification, Characterization, and Steady-State Kinetics of a meta-Cleavage Compound Hydrolase from Pseudomonas fluorescens IP01
- Expression Pattern of the CsPK3 Auxin-Responsive Protein Kinase Gene
- Purification and Properties of an Amine Dehydrogenase from Pseudomonas K95 grown on 1,12-Diaminododecane (DAD)(Microbiology & Fermentation Industry)
- Studies on Fermentation Products and Metabolism of Long Chain Alkyl Diamine in Microorganisms(Microbiology & Fermentation Industry)
- A New Metabolic Pathway for meta Ring-fission Compounds of Biphenyl(Biological Chemistry)
- Purification and Some Properties of 2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid(HOPDA) Reducing Enzyme from Pseudomonas cruciviae S93B1 Involved in the Degradation of Biphenyl(Biological Chemistry)
- Microbial Dehalogenation of Haloalkanes Mediated by Oxygenase or Halidohydrolase
- Formation of 2, 4, 6-Trihydroxy-carboxylic Acid and 2-Proto-catechuoylphlorogmcinol-carboxylic Acid from Rutin by Bacteria
- Microbial Degradation Products from Biphenyl-related Compounds
- Enzymatic Dioxygenation of Biphenyl-2, 3-diol and S-Isopropylcatechol
- Purification and Some Properties of a 2-Hydroxy-6-oxo-6-phenylhexa-2, 4-dienoic Acid Hydrolyzing Enzyme from Pseudomonas cruciviae S93 Bl involved in the Degradation of Biphenyl
- Detection of a Bacterial Group within the Phylum Chloroflexi and Reductive-Dehalogenase-Homologous Genes in Pentachlorobenzene-Dechlorinating Estuarine Sediment from the Arakawa River, Japan
- Genes Involved in Aniline Degradation by Delftia acidovorans Strain 7N and Its Distribution in the Natural Environment
- Isolation and Characterization of an Alkaliphilic Bacterium Utilizing Pyrene as a Carbon Source(ENVIRONMENTAL BIOTECHNOLOGY)
- Divergent Structures of Carbazole Degradative car Operons Isolated from Gram-negative Bacteria
- Rhizoremediation of Dioxin-like Compounds by a Recombinant Rhizobium tropici Strain Expressing Carbazole 1,9a-Dioxygenase Constitutively(Microbiology & Fermentation Technology)
- Expression, Purification, and Characterization of 2'-Aminobiphenyl-2,3-diol 1,2-dioxygenase from Carbazole-degrader Pseudomonas resinovorans Strain CA10(Microbiology & Fermentation Technology)
- Purification and Characterization of meta-Cleavage Compound Hydrolase from a Carbazole Degrader Pseudomonas resinovorans Strain CA10(Microbiology & Fermentation Technology)
- Cloning and Characterization of cDNAs for the Jasmonic Acid-responsive Genes RRJ1 and RRJ2 in Suspension-cultured Rice Cells(Biochemistry & Molecular Biology)
- Organization and Transcriptional Characterization of Catechol Degradation Genes Involved in Carbazole Degradation by Pseudomonas resinovorans Strain CA10(Microbiology & Fermentation Technology)
- Evidence for the Presence of DNA-Binding Proteins Involved in Regulation of the Gene Expression of Indole-3-Pyruvic Acid Decarboxylase, a Key Enzyme in Indole-3-Acetic Acid Biosynthesis in Azospirillum lipoferum FS
- New Classification System for Oxygenase Components Involved in Ring-Hydroxylating Oxygenations
- Isolation and Characterization of Low-indole-3-acetic Acid-producing Mutants from Bradyrhizobium elkanii
- Polypeptide Requirement of Multicomponent Monooxygenase DsoABCDEF for Dimethyl Sulfide Oxidizing Activity
- Cloning and Characterization of the Genes Involved in the Degradation of Dibenzofuran by Terrabacter sp. Strain DBF63
- Cloning and Nucleotide Sequences of the Genes Involved in the meta-Cleavage Pathywa of Cumene Degradation in Pseudomonas fluorescens IP01
- Cloning, Nucleotide Sequence, and Characterization of the Genes Encoding Enzymes Involved in the Degradation of Cumene to 2-Hydroxy-6-Oxo-7-Methylocta-2,4-Dienoic Acid in Pseudomonas fluorescens IP01
- Diterpene Phytoalexins Are Biosynthesized in and Exuded from the Roots of Rice Seedlings
- Involvement of the Basic Helix-Loop-Helix Transcription Factor RERJ1 in Wounding and Drought Stress Responses in Rice Plants
- Preparation and Biological Activity of Molecular Probes to Identify and Analyze Jasmonic Acid-binding Proteins
- 1P-023 芳香環水酸化ジオキシゲナーゼの電子伝達コンポーネント(ferredoxin reductase)のX線結晶構造解析(蛋白質・構造(1),第46回日本生物物理学会年会)
- Two-stage electrodialytic concentration of glyceric acid from fermentation broth(BIOCHEMICAL ENGINEERING)
- Phylogenetic Analyses of Bacterial Communities Developed in a Cassette-Electrode Microbial Fuel Cell
- Production of Glyceric Acid by Gluconobacter sp. NBRC3259 Using Raw Glycerol
- CLONING OF ent-KAURENE SYNTHASE B cDNA FROM PUMPKIN(Cucurbita maxima L. )
- Elicitor and Calatalse Activity of Conidia Suspensions of Various Strains of Magnaporthe grisea in Suspension-Cultured Cells of Rice
- Induction of Resistance against Rice Blast Fungus in Rice Plants Treated with a Potent Elicitor, N-Acetylchitooligosaccharide
- Characterization of an Elicitor-Induced Rice WRKY Gene, OsWRKY71
- Diterpene Cyclases Responsible for the Biosynthesis of Phytoalexins, Momilactones A, B, and Oryzalexins A-F in Rice
- Isolation and Identification of Antheridiogens in the Ferns, Lygodium microphyllum and Lygodium reticulatum(Organic Chemistry)
- Bacterial Lipopolysaccharides Induce Defense Responses Associated with Programmed Cell Death in Rice Cells
- cDNA CLONING OF ent-KAURENE SYNTHETASEA FROM RICE
- Identification of Gibberellins and 9,15-Cyclogibberellins in Developing Apple Seeds
- Gibberellink and Antheridiogens in Prothallia and Sporophytes of Anemia phyllitidis
- Use of a Gluconobacter frateurii Mutant to Prevent Dihydroxyacetone Accumulation during Glyceric Acid Production from Glycerol
- Application of electrodialysis to glycerate recovery from a glycerol containing model solution and culture broth(BIOCHEMICAL ENGINEERING)
- Glycerol Conversion to D-Xylulose by a Two-stage Microbial Reaction Using Candida parapsilosis and Gluconobacter oxydans
- Convenient Transformation of Anamorphic Basidiomycetous Yeasts Belonging to Genus Pseudozyma Induced by Electroporation(METHODS)
- Effects of Fluorogibberellins on Plant Growth and Gibberellin 3β-Hydroxylases
- Inhibition of ent-Kaurene Synthase by Quaternary Ammonium Growth Retardants
- Effects of Gibberellins and Prohexadione on the Activities of Oryzain and α-Amylase in Rice Seeds : PROTEINS, ENZYMES AND METABOLISM
- Characterization of a Rice Gene Family Encoding Type-A Diterpene Cyclases
- A Step in the Biosynthesis of Gibberellins that Is Controlled by the Mutation in the Semi-Dwarf Rice Cultivar Tan-Ginbozu
- Structural and Functional Characterization of Oryzacystatin-I Monomer and Dimer
- cDNA Cloning and Characterization of Gibberellin-responsive Genes in Photoblastic Lettuce Seeds
- Effects of Exogenously Applied Gibberellin and Red Light on the Endogenous Levels of Abscisic Acid in Photoblastic Lettuce Seeds : GROWTH AND DEVELOPMENT
- Characterization of a Protein Kinase Gene Responsive to Auxin and Gibberellin in Cucumber Hypocotyls
- Qualitative and Quantitative Analysis of Endogenous Gibberellins in Raphanus sativus L. during Cold Treatment and the Subsequent Growth
- Alteration of the Substrate Specificity of the Angular Dioxygenase Carbazole 1,9a-Dioxygenase
- Endocrine disruptors that disrupt the transcription mediated by androgen receptor
- Biodegradation of Carbazole by Pseudomonas spp. CA06 and CA10
- Preparation of (±)-2-(2,3-^2H_2)Jasmonic Acid and Its Methyl Ester, Methyl (±)-2-(2,3-^2H_2)Jasmonate
- Behavior of Various Hosts of the IncP-7 Carbazole-Degradative Plasmid pCAR1 in Artificial Microcosms
- The Behavior and Significance of Degradative Plasmids Belonging to Inc Groups in Pseudomonas within Natural Environments and Microcosms
- The Complete Nucleotide Sequence of pCAR2: pCAR2 and pCAR1 Were Structurally Identical IncP-7 Carbazole Degradative Plasmids
- Cloning and Characterization of cDNAs Encoding ent-Copalyl Diphosphate Synthases in Wheat: Insight into the Evolution of Rice Phytoalexin Biosynthetic Genes
- Comparison of the Enzymatic Properties of ent-Copalyl Diphosphate Synthases in the Biosynthesis of Phytoalexins and Gibberellins in Rice
- Genetic Evidence for the Role of Isopentenyl Diphosphate Isomerases in the Mevalonate Pathway and Plant Development in Arabidopsis
- 4-O-Caffeoylshikimic and 4-O-(p-Coumaroyl)shikimic Acids from the Dwarf Tree Fern, Dicksonia antarctica
- Bioactive Gibberellin and Its Metabolism in Shoots of Phaseolus vulgaris
- Molecular Bases of Aerobic Bacterial Degradation of Dioxins : Involvement of Angular Dioxygenation
- Involvement of Gibberellins in Expression of a Cysteine Proteinase (SH-EP) in Cotyledons of Vigna mungo Seedlings
- POSSIBLE INVOL VEMENT OF GIBBERELLINS IN EXPRESSION OF PROTEINASE ACTIVITY IN COTYLEDONS OF Vigna mungo SEEDLINGS
- Bioprocessing of Glycerol into Glyceric Acid for Use in Bioplastic Monomer
- Synthesis and Evaluation of Dioleoyl Glyceric Acids Showing Antitrypsin Activity
- Microbial Degradation of the Polycyclic Aromatic Hydrocarbons Acenaphthene and Acenaphthylene by a Pure Bacterial Culture
- Promoter Analysis of the Elicitor-Induced WRKY Gene OsWRKY53, Which Is Involved in Defense Responses in Rice
- Alteration of the Substrate Specificity of the Angular Dioxygenase Carbazole 1,9a-Dioxygenase
- Elicitor and Calatalse Activity of Conidia Suspensions of Various Strains of Magnaporthe grisea in Suspension-Cultured Cells of Rice
- Diterpene Phytoalexins Are Biosynthesized in and Exuded from the Roots of Rice Seedlings
- Comparison of the Enzymatic Properties of ent-Copalyl Diphosphate Synthases in the Biosynthesis of Phytoalexins and Gibberellins in Rice
- cDNA Cloning and Characterization of a Gibberellin-Responsive Gene in Hypocotyls of Cucumis sativus L.
- Cloning and Characterization of cDNAs Encoding ent-Copalyl Diphosphate Synthases in Wheat : Insight into the Evolution of Rice Phytoalexin Biosynthetic Genes
- Characterization of an Elicitor-Induced Rice WRKY Gene, OsWRKY71
- Use of a Gluconobacter frateurii Mutant to Prevent Dihydroxyacetone Accumulation during Glyceric Acid Production from Glycerol
- Identification of the Electron Transfer Flavoprotein as an Upregulated Enzyme in the Benzoate Utilization of Desulfotignum balticum
- Identification of the Electron Transfer Flavoprotein as an Upregulated Enzyme in the Benzoate Utilization of Desulfotignum balticum
- The Behavior and Significance of Degradative Plasmids Belonging to Inc Groups in Pseudomonas within Natural Environments and Microcosms
- Behavior of Various Hosts of the IncP-7 Carbazole-Degradative Plasmid pCAR1 in Artificial Microcosms
- The Complete Nucleotide Sequence of pCAR2 : pCAR2 and pCAR1 Were Structurally Identical IncP-7 Carbazole Degradative Plasmids
- Production of Glyceric Acid by Gluconobacter sp. NBRC3259 Using Raw Glycerol
- Identification of a Jasmonic Acid-Responsive Region in the Promoter of the Rice 12-Oxophytodienoic Acid Reductase 1 Gene OsOPR1
- Gravitational Lens Effect on the Cosmic Background Radiation Due to Nonlinear Inhomogeneities : Astrophysics and Relativity
- Oligomerization and DNA-Binding Capacity of Pmr, a Histone-Like Protein H1 (H-NS) Family Protein Encoded on IncP-7 Carbazole-Degradative Plasmid pCAR1
- Functional Identification of a Rice ent-Kaurene Oxidase, OsKO2, Using the Pichia pastoris Expression System
- Suppressive effect of abscisic acid on systemic acquired resistance in tobacco plants
- Membrane Topology and Functional Analysis of Methylobacillus sp. 12S Genes epsF and epsG, Encoding Polysaccharide Chain-Length Determining Proteins
- OsJAR1 Contributes Mainly to Biosynthesis of the Stress-Induced Jasmonoyl-Isoleucine Involved in Defense Responses in Rice