Comparison of the Enzymatic Properties of ent-Copalyl Diphosphate Synthases in the Biosynthesis of Phytoalexins and Gibberellins in Rice
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概要
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The rice genome contains two ent-copalyl diphosphate synthase genes: OsCPS1 acts in gibberellin (phytohormone) biosynthesis, and OsCPS2/OsCyc2 acts in the synthesis of oryzalexins A-F and phytocassanes A-E (phytoalexins). We characterized the enzymatic properties of recombinant OsCPS2/OsCyc2 fused with a tag-protein at the N-terminus, and compared them to those of OsCPS1. Several enzymatic properties of OsCPS2/OsCyc2, including the optimal pH, optimal temperature, divalent cation requirement, and kinetic values for the geranylgeranyl diphosphate (GGDP) substrate, were almost the same as those of OsCPS1. However, OsCPS2/OsCyc2 activity was not inhibited by 50–60 μM GGDP substrate, by which the OsCPS1 activity was inhibited. Furthermore, the OsCPS1 activity exhibited approximately 70% inhibition by 100 μM Amo-1618 (a gibberellin biosynthetic inhibitor), whereas the OsCPS2/OsCyc2 activity exhibited approximately 10% inhibition. These results indicate that the properties of OsCPS2/OsCyc2 were partially different from those of OsCPS1, although OsCPS2/OsCyc2 catalyzes the same reaction step as OsCPS1.
- 社団法人 日本農芸化学会の論文
著者
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YAMANE Hisakazu
Biotechnology Research Center, The University of Tokyo
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HIROSE Yuko
Department of Chemistry, Faculty of Education and Human Sciences, University of Yamanashi
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Toyomasu Tomonobu
Department of Bioresource Engineering, Yamagata University
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MITSUHASHI Wataru
Department of Bioresource Engineering, Yamagata University
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SASSA Takeshi
Department of Bioresource Engineering, Yamagata University
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DAIRI Tohru
Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University
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HAYASHI Yutaka
Biotechnology Research Center, Toyama Prefectural University
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ONODERA Yu
Department of Bioresource Engineering, Yamagata University
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