Alteration of the Substrate Specificity of the Angular Dioxygenase Carbazole 1,9a-Dioxygenase
スポンサーリンク
概要
- 論文の詳細を見る
Carbazole 1,9a-dioxygenase (CARDO) consists of terminal oxygenase (CARDO-O) and electron transport components. CARDO can catalyze specific oxygenation for various substrates: angular dioxygenation for carbazole and dibenzo-p-dioxin, lateral dioxygenation for anthracene, and monooxygenation for methylene carbon of fluorene and sulfide sulfur of dibenzothiophene. To elucidate the molecular mechanism determining its unique substrate specificity, 17 CARDO-O site-directed mutants at amino acid residues I262, F275, Q282, and F329, which form the substrate-interacting wall around the iron active site by CARDO-O crystal structure, were generated and characterized. F329 replacement dramatically reduced oxygenation activity. However, several mutants produced different products from the wild-type enzyme to a large extent: I262V and Q282Y (1-hydroxycarbazole), F275W (4-hydroxyfluorene), F275A (unidentified cis-dihydrodiol of fluoranthene), and I262A and I262W (monohydroxydibenzothiophenes). These results suggest the possibility that the respective substrates bind to the active sites of CARDO-O mutants in a different orientation from that of the wild-type enzyme.
- 社団法人 日本農芸化学会の論文
著者
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FURIHATA Kazuo
Department of Applied Biological Chemistry, The University of Tokyo
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NOJIRI Hideaki
Biotechnology Research Center, The University of Tokyo
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YAMANE Hisakazu
Biotechnology Research Center, The University of Tokyo
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OMORI TOSHIO
Biotechnology Research Center, The University of Tokyo
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HABE HIROSHI
Biotechnology Research Center, The University of Tokyo
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TAKEMURA TETSUO
Department of Chemistry, Faculty of Sciences, Science University of Tokyo
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NAKAMURA Shugo
Department of Biotechnology, The University of Tokyo
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Usami Yusuke
Biotechnology Research Center, The University of Tokyo
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Uchimura Hiromasa
Biotechnology Research Center, The University of Tokyo
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Noguchi Haruko
Biotechnology Research Center, The University of Tokyo
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HORISAKI Tadafumi
Biotechnology Research Center, The University of Tokyo
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UMEDA Takashi
Biotechnology Research Center, The University of Tokyo
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LI Li
Biotechnology Research Center, The University of Tokyo
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TERADA Tohru
Professional Program for Agricultural Bioinformatics, The University of Tokyo
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SHIMIZU Kentaro
Professional Program for Agricultural Bioinformatics, The University of Tokyo
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Horisaki Tadafumi
Dep. Of Chemistry Fac. Of Sci. Tokyo Univ. Of Sci.
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