Inhibitory Effect of the Conformation of Amylose as a Function of I_2 Concentration on Glucoamylase Activity
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概要
- 論文の詳細を見る
In order to gain a better understanding of the adaptability of glucoamylase to the conformational change in the substrate related with the specificity of the enzyme action, the effect of the solution conformation of amylose (the substrate) as a function of the I_2 concentration on enzyme activity was studied in the initial state by using amyloses with a wide range of average degree of polymerization (DP^^^-) in the presence of excess KI. The enzyme activity for the amylose oligomers in the (DP^^^-) range of 20 to 100 decreased monotonically with increasing I_2 concentration. This inhibition of the enzyme activity is ascribed to the effect of the conformational change in amylose of the random coil or wormlike chain to a helix that is induced by the binding of I^-_3ions responsible for inclusion complexation in combination with direct effect of free I^-_3ions. In a rapidly mixed complex system for amylose with a large (DP^^^-) of 1,000,however, a significant two-step form of the inhibition of enzyme activity appeared with increasing I_2 concentration, corresponding to the two-step conformational change of amylose in a random coil or worm-like chain to a helix and then to a rod-like compact structure.
- 社団法人日本農芸化学会の論文
- 1999-06-23
著者
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TAKEMURA TETSUO
Department of Chemistry, Faculty of Sciences, Science University of Tokyo
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Yajima Hirofumi
Department of Applied Chemistry, Faculty of Science, Tokyo University of science
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Yajima H
Department Of Applied Chemistry Faculty Of Science Tokyo University Of Science
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Yajima Hirofumi
Department Of Applied Chemistry Faculty Of Science Tokyo University Of Science
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Yajima Hirofumi
Department Of Applied Chemistry Faculty Of Science Science University Of Tokyo
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Terada Tohru
RIKEN
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ISHII Tadahiro
Department of Applied Chemistry, Faculty of Science, Science University of Tokyo
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Ishii T
Sumitomo Chemical Co. Ltd. Takarazuka Jpn
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Ishii Tadahiro
Science University Of Tokyo Faculty Of Science
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Ishii Tadahiro
Department Of Applied Chemistry Faculty Of Science Science University Of Tokyo
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Terada Tohru
Agricultural Bioinformatics Reserch Unit Graduate School Of Agricultural And Life Sciences Univ. Of
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WATANABE Kazuo
Nippi Research Institute of Biomatrix
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Takemura T
Agricultural Bioinformatics Research Unit Graduate School Of Agricultural And Life Sciences The Univ
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Takemura Tetsuo
Department Of Applied Physics Faculty Of Engineering Kyushu University
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Terada Tohru
Agricultural Bioinformatics Reserch Unit Graduate School Of Agricultural And Life Sciences Univ. Of
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Terada Tohru
Agricultural Bioinformatics Reserch Unit Graduate School Of Agricultural And Life Sciences Univ. Of
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