Isolation and Genetic Characterization of pGKL Killer-insensitive Mutants (iki) from Saccharomyces cerevisiae

スポンサーリンク

概要

• 論文の詳細を見る

• The linear double stranded DNA plasmid pGKL1 encodes the yeast killer toxin complex (Gunge et al., 1981) of which the killing mechanism is not understood. We isolated and characterized eight mutants in Saccharomyces cerevisiae that were insensitive to both the intracellularly expressed 28-kDa killer subunit and the native killer toxin complex. These mutations (iki1 through iki5) were all recessive, and classified into five complementation groups. The iki2 mutation was mapped to a position near the centromere on chromosome XIII. We developed a novel screening system to isolate the DNA fragments complementing the iki mutations from a Sccharomyces gene library, and isolated three DNA fragments that complement the iki1, iki3, and iki4 mutations, respectively.

• 社団法人日本農芸化学会の論文
• 1996-05-23

著者

• Kishida Masao

  Center For Research And Development Of Bioresources University Of Osaka Prefecture

• Kishida Masao

  Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture University

• Kishida M

  Department Of Applied Biochemistry Faculty Of Agriculture Osaka Prefecture University

• Kawamura‐watabe A

  Mitsubishi Kasei Inst. Life Sci. Tokyo Jpn

• Kawamura-watabe Akiko

  Mitsubishi Kasei Institute Of Life Sciences

• Yajima H

  Department Of Applied Chemistry Faculty Of Science Tokyo University Of Science

• Yajima Hirofumi

  Department Of Applied Chemistry Faculty Of Science Tokyo University Of Science

• HISHINUMA FUMIO

  Mitsubishi Kasei Institute of Life Science

• Tokunaga Masao

  Faculty of Agri., Kagoshima Univ.

• Kishida Masao

  Mitsubishi Kasei Institute Of Life Sciences

• Tokunaga M

  Applied And Molecular Microbiology Faculty Of Agriculture Kagoshima University

• YAJIMA Hirohiko

  Mitsubishi Kasei Institute of Life Sciences

• Katayose Yuichi

  Mitsubishi Kasei Institute Of Life Sciences

• Hishinuma F

  Mitsubishi Kasei Inst. Life Sci. Tokyo Jpn

• Tokunaga Masao

  Faculty Of Agri. Kagoshima Univ.

関連論文

• 2P009 Halomonas sp.593由来ヌクレオシドニリン酸キナーゼ(HaNDK)の多量体構造(蛋白質-構造,第48回日本生物物理学会年会)
• Chromosomal Deletion or Rearrangement in Chimeric Hybrids of Saccharomycopsis fibuligera and Saccharomyces diastaticus Obtained by Cell Fusion
• Introduction of Mitochondria into Respiratory-Deficient Mutant of Yeast and Improvement of Thermostability
• Breeding a Fermentative Yeast at High Temperature Using Protoplast Fusion
• FM-P46 Optimization of bacteriocin production by thermotolerant lactic acid bacteria, Pediococcus KKU 170, isolated from Thai local fermented food(Section VIII Fermentation and Microbial Technology)
• Identification of an N-acetylcysteine conjugate in the urine after oral administration of 2,4-dichloro-1-nitrobenzene to rats
• Optimization of the Expression of a Foreign Gene from Galactose-Inducible Promoter in a Recombinant Yeast
• 1P030 Homodimeric Crossover Structure of the Human GCSF-Receptor Signaling Complex(1. Protein structure and dynamics (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• Expression and Purification of the Bacillus subtilis Thioredoxin Superfamily Protein YkvV
• Molecular Cloning of groESL Locus, and Purification and Characterization of Chaperonins, GroEL and GroES, from Bacillus brevis
• Secretion of Hen Egg White Lysozyme from Kluyveromyces lactis
• Expression and Purification of Cytokine Receptor Homology Domain of Human Granulocyte-Colony Stimulating Factor Receptor in Escherichia coli
• Purification and Characterization of an Endo-Polygalacturonase from a Mutant of Saccharomyces cerevisiae
• Isolation and Genetic Determination of a Saccharomyces cerevisiae Mutant That Produces an Endo-Polygalacturonase
• Molecular Cloning and Expression of a Polygalacturonase Gene in Saccharomyces cerevisiae
• Characterization of Hydroxy-biphenyl-O-sulfates in Urine and Urine Crystals Induced by Biphenyl and KHCO_3 Administration in Rats
• Characterization of IKI1 and IKI3 Genes Conferring pGKL Killer Sensitivity on Saccharomyces cerevisiae
• Inhibitory Effect of the Conformation of Amylose as a Function of I_2 Concentration on Glucoamylase Activity
• Purification and Characterization of Three Extracellular Protopectinases with Poly-galacturonase Activities from Trichosporon penicillatum
• Regioselective Synthesis of Prenylisoflavones: Syntheses of Luteone and Luteone Hydrate
• Characterization of Nucleoside Diphosphate Kinase from Moderately Halophilic Eubacteria(Microbiology & Fermentation Technology)
• Isolation and Genetic Characterization of pGKL Killer-insensitive Mutants (iki) from Saccharomyces cerevisiae
• A Cold-Sensitive spo14 Mutation Affecting Ascospore Formation in the Fission Yeast Schizosaccharomyces pombe
• Rapid Detection of Acetic Acid Bacteria in the Traditional Pot-Fermented Rice Vinegar Kurozu
• Isolation and Nucleotide Sequence Analysis of the URA3 (orotidine 5'-phosphate decarboxylase) Gene of Kluyverimyces lactis(Microbiology & Fermentation Industry)
• Isolation and Characterization of kar2-404 Mutation in Saccharomyces cerevisiae
• Effect of chirality by the free electron laser irradiation (特集 機能性有機薄膜・複合膜とデバイス応用)
• 2G1624 Chromohalobacter sp.560由来β-LactamaseのX線結晶解析(蛋白質_構造2,第49回日本生物物理学会年会)
• Construction of a Plasmid Replicating in Both Saccharomyces cerevisiae and Kluyveromyces lactis
• Intact Cell Transformation of Saccharomyces cerevisiae by Polyethylene Glycol

もっと見る 閉じる

スポンサーリンク