Secretion of Hen Egg White Lysozyme from Kluyveromyces lactis

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概要

• 論文の詳細を見る

• Hen egg white (HEW) lysozyme was correctly processed and efficiently secreted from an alternative yeast, Kluyveromyces lactis. We constructed secretion vectors using PHO5,PGK, and LAC4 promoters, and found that the highest secretion was obtained under the direction of the PGK promoter in non-selective rich medium. K. lactis secreted HEW lysozyme with twofold higher efficiency than S. cerevisiae, estimated by using a K. lactis-S. cerevisiae shuttle vector.

• 社団法人日本農芸化学会の論文
• 2000-12-23

著者

• HIRAI TAKANORI

  Komaki Municipal Hospital

• Tanaka Ryoichi

  Faculty Of Agriculture Kagoshima University

• Takagi H

  Department Of Food Science And Technology Faculty Of Agriculture Nagoya University:(present Address)

• Takagi Hiroshi

  Second Department Of Surgery Nagoya University

• TOKUNAGA Masao

  Laboratory of Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University

• TOKUNAGA Hiroko

  Laboratory of Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University

• ISHIBASHI Matsujiro

  Laboratory of Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University

• TANAKA Ryoichi

  Laboratory of Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University

• Takagi H

  Department Of Bioscience Fukui Prefectural University

• Tokunaga M

  Applied And Molecular Microbiology Faculty Of Agriculture Kagoshima University

• Tokunaga Masao

  Laboratories Of Molecular Genetics Mitsubishi Kasei Institute Of Life Sciences

• Tanaka Ryutaro

  Faculty Of Agriculture Kagoshima University

• Tokunaga Hiroko

  Laboratory Of Applied And Molecular Microbiology Faculty Of Agriculture Kagoshima University

• Takagi H

  Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.

• Ishibashi Matsujiro

  Applied And Molecular Microbiology Faculty Of Agriculture Kagoshima University

• Tokunaga Masao

  Faculty Of Agri. Kagoshima Univ.

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