Chemical Synthesis of the Gene for Microbial Transglutaminase from Streptoverticillium and Its Expression in Escherichia coli
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概要
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The gene coding for microbial transglutaminase (TGase) from Streptoverticillium, which consists of 331 amino acids, was chemically synthesized. The codons have been substituted for those mainly favored in yeast. Our strategy involved the construction of the TGase gene in five sections (54 oligomers) that contained unique restriction enzyme sites at both ends, which could readily be ligated to form the full-length product. The chemically synthesized gene was inserted downstream from the ompA signal peptide of the E. coli expression vector, pIN-III-ompA, which carries lpp and lac promotors. The resultant plasmid directed the expression of TGase, with the activity being secreted mainly into the periplasmic space of E. coli. The induced gene product was identical with native TGase in size and in immunological properties, though the enzyme activity was low.
- 社団法人日本農芸化学会の論文
- 1994-01-23
著者
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MOTOKI Masao
Food Research & Development Laboratories, Ajinomoto Co., Inc.
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Ando K
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Takagi H
Department Of Food Science And Technology Faculty Of Agriculture Nagoya University:(present Address)
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Motoki M
Ajinomoto Co. Inc. Kawasaki Jpn
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Takeuchi Kazuyuki
Research And Development Division Amano Prarmaceutical Co. Ltd.
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Takeuchi Kazuyuki
Tsukuba Research Laboratories Amano Pharmaceutical Co. Ltd.
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Takagi H
Fukui Prefectural Univ. Fukui Jpn
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Takehana Shino
Food Research & Development Laboratories of Ajinomoto Co., Inc.
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Washizu Kinya
Food Research & Development Laboratories of Ajinomoto Co., Inc.
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Ando Keiichi
Tsukuba Research Laboratories of Amano Pharmaceutical Co., Ltd.
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Koikeda Satoshi
Tsukuba Research Laboratories of Amano Pharmaceutical Co., Ltd.
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Matsui Hiroshi
Tsukuba Research Laboratories of Amano Pharmaceutical Co., Ltd.
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Takagi Hiroshi
Food Research & Development Laboratories of Ajinomoto Co., Inc.
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Washizu Kinya
Tsukuba Research Laboratories, Amano Pharmaceutical Co., Ltd.
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Washizu Kinya
Tsukuba Research Laboratories Amano Pharmaceutical Co. Ltd.
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Takehana Shino
Food Research & Development Laboratories Of Ajinomoto Co. Inc.
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Koikeda Satoshi
Tsukuba Research Laboratories Amano Pharmaceutical Co. Ltd.
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Ando K
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd
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Takagi H
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.
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Matsui Hiroshi
Tsukuba Research Laboratories Of Amano Pharmaceutical Co. Ltd.
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