Molecular Cloning of groESL Locus, and Purification and Characterization of Chaperonins, GroEL and GroES, from Bacillus brevis

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概要

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• The groESL locus of a protein-hypersecreting bacterium, Bacillus brevis, was cloned by PCR using primers designed based on the DNA sequence of a B. subtilis homolog. GroEL protein was purified to apparent homogeneity and its ATPase activity was characterized : it hydrolyzed ATP, CTP, and TTP in this order of reaction rate, and its specific activity for ATP was 0.1μmole/min/mg protein. Purified GroEL forms a tetradecamer. GroEL was estimated to contain 22% α-helix, 24% β-sheet, and 19% turn structures, by CD measurement. GroES protein was also highly purified to examine its chaperonin activity. GroEL protected from thermal inactivation of and showed refoldingpromoting activity for malate dehydrogenase, strictly depending on the presence of ATP and GroES.

• 社団法人日本農芸化学会の論文
• 2001-06-23

著者

• HIRAI TAKANORI

  Komaki Municipal Hospital

• Tanaka Ryoichi

  Faculty Of Agriculture Kagoshima University

• Takagi H

  Department Of Food Science And Technology Faculty Of Agriculture Nagoya University:(present Address)

• Takagi Hiroshi

  Second Department Of Surgery Nagoya University

• SHIRAISHI Yoichi

  the Department of Computer Science, Faculty of Engineering, Gunma University

• MIZUKAMI Makoto

  Department of Research and Development, Higeta Shoyu Co.

• TOKUNAGA Masao

  Laboratory of Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University

• SHIRAISHI Yoichi

  Laboratory of Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University

• ODACHI Masatake

  Laboratory of Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University

• TOKUNAGA Hiroko

  Laboratory of Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University

• PHILO John

  Alliance Protein Laboratories, 3957 Corte Cancion, Thousand Oaks

• ARAKAWA Tsutomu

  Alliance Protein Laboratories, 3957 Corte Cancion, Thousand Oaks

• ISHIBASHI Matsujiro

  Laboratory of Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University

• TANAKA Ryoichi

  Laboratory of Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University

• TAKAGI Hiroaki

  Department of Research & Development, Higeta Shoyu Co.

• Shiraishi Yoichi

  Laboratory Of Applied And Molecular Microbiology Faculty Of Agriculture Kagoshima University

• Takagi Hiroaki

  Department Of Food Science And Technology Faculty Of Agriculture Nagoya University:(present Address)

• Takagi H

  Department Of Bioscience Fukui Prefectural University

• Tokunaga M

  Applied And Molecular Microbiology Faculty Of Agriculture Kagoshima University

• Tokunaga Masao

  Laboratories Of Molecular Genetics Mitsubishi Kasei Institute Of Life Sciences

• Tanaka Ryutaro

  Faculty Of Agriculture Kagoshima University

• Philo John

  Alliance Protein Laboratories 3957 Corte Cancion Thousand Oaks

• Arakawa T

  Alliance Protein Laboratories

• Arakawa Tsutomu

  Alliance Protein Lab.

• Tokunaga Hiroko

  Laboratory Of Applied And Molecular Microbiology Faculty Of Agriculture Kagoshima University

• Takagi H

  Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.

• Arakawa T

  Allaiance Protein Laboratories

• Odachi Masatake

  Laboratory Of Applied And Molecular Microbiology Faculty Of Agriculture Kagoshima University

• Ishibashi Matsujiro

  Applied And Molecular Microbiology Faculty Of Agriculture Kagoshima University

• Mizukami M

  Department Of Research And Development Higeta Shoyu Co.

• Mizukami Makoto

  Department Of Food Science And Technology Faculty Of Agriculture Nagoya University:nihon Shokuhin Ka

• Tokunaga Masao

  Faculty Of Agri. Kagoshima Univ.

• PHILO John

  Alliance Protein Laboratories

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