Efficient Production of Thermostable Clostridium thermosulfurogenes β-Amylase by Bacillus brevis

概要

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The Bacillus brevis host-vector system was used for production of the thermostable Clostridium thermosulfurogenes β-amylase. The promoter and translation initiation region of the cell wall protein gene operon (cwp) of B. brevis were used to express the β-amylase gene in B. brevis 47. B. brevis 47K, a previously isolated mutant that secerted human a-amylase efficiently was shown to be also a good host for the β-amylase production. When the Clostridium signal peptide was used to direct secretion, 0.3 g of the β-amylase with a correct NH_2-terminus was secerted per liter of medium. When the signal peptide of a B. brevis cell wall protein was used, a very large amount (1.6 g/l) of the enzyme was secreted, which is about 60 times larger than that secreted by C. thermosulfurogenes, but the NH_2-terminus of the secreted enzyme was heterogeneous. Both enzymes secreted by B. brevis showed almost the same specific activity, thermostability, and ability to bind and to digest raw starch, as those of the C. thermosulfurogenes enzyme.
社団法人日本生物工学会の論文
1992-02-25