Xylose (Glucose) Isomerase Gene from the Thermophile Clostridium thermohydrosulfuricum; Cloning, Sequencing, and Expression in Escherichia coli(Microbiology & Fermentation Industry)

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概要

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• The xylose isomerase gene from the thermophile Clostridium thermohydrosulfuricum has been cloned, using a fragment of the Bacillus subtilis gene as a probe. The complete nucleotide sequence of the gene was analyzed. C. thermohydrosulfuricum is the most thermophilic organism from which a xylose isomerase gene has been cloned and characterized. Comparison with amino acid sequences from other xylose isomerases showed that amino acids involved in substrate binding and isomerization are well conserved. Purification of the enzyme produced in E. coll was done by heating a cell-free extract at 85℃ for 10min, giving a 20-fold purified enzyme. The native enzyme is a homomeric tetramer with a molecular weight of 200,000.

• 社団法人日本農芸化学会の論文
• 1991-01-23

著者

• Dekker Koen

  Faculty Of Agriculture Nagoya University:nihon Shokuhin Kako Co. Ltd.

• Udaka S

  Faculty Of Applied Bio-science Tokyo University Of Agriculture

• Udaka Shigezo

  Faculty Of Agriculture Nagoya University:department Of Brewing And Fermentation Tokyo University Of

• Yamagata Hideo

  Department Of Food Science And Technology Facult Of Agriculture Nagoya University

• YAMAGATA Hideo

  Nihon Shokuhin Kako Co., Ltd.

• SAKAGUCHI Kenji

  Faculty of Agriculture, Nagoya University

• Sakaguchi Kenji

  Research Institute For Food Science Kyoto University

• Udaka Shigezo

  Dept. Of Food Science And Technology Faculty Of Agriculture Nagoya University

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