Efficient Production of Casoxin D, a Bradykinin Agonist Peptide Derived from Human Casein, by Bacillus brevis
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概要
- 論文の詳細を見る
We efficiently produced a small peptide by the host-vector system using Bacillus brevis as a host. DNA encoding the physiologically functional casoxin D, composed of seven amino acids, was ligated in tandem. An expression-secretion vector containing DNA, which codes for a fusion protein of epidermal growth factor-casoxin D pentamer, was constructed. B. brevis transformed with this plasmid produced about 0.5g/liter of the fusion protein in the culture supernatant. The fusion protein was puritied with ammonium sulfate fractionation from the supernatant and digested with two kinds of proteinases. A peptide well separated by high pressure liquid chromatography was identified as biologically active casoxin D.
- 社団法人日本農芸化学会の論文
- 1995-11-23
著者
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YOSHIKAWA Masaaki
Faculty of Science, Hokkaido University
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Chiba H
Faculty Of Domestic Science Kobe Women's University
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Chiba Hideo
Faculty Of Domestic Science Kobe Women's University
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Udaka Shigezo
Faculty Of Agriculture Nagoya University:department Of Brewing And Fermentation Tokyo University Of
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Fujiwara Yuko
Faculty Of Agriculture Nagoya University
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Okamoto Akiko
Department Of Organic And Polymer Materials Chemistry Tokyo University Of Agriculture And Technology
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Kato Masashi
Faculty of Agriculture, Nagoya University
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Okamoto Akiko
Department Of Brewing And Fermentation Tokyo University Of Agriculture
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Yoshikawa Masaaki
Faculty Of Agriculture Kyoto University
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Kato Masashi
Faculty Of Agriculture Nagoya University
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Okamoto Akiko
Department of Allergy and Rheumatology, Graduate School of Medicine, the University of Tokyo
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