Expression and Purification of the Bacillus subtilis Thioredoxin Superfamily Protein YkvV
スポンサーリンク
概要
- 論文の詳細を見る
Bacillus subtilis YkvV protein, an extracellular thioredoxin superfamily protein, was successfully expressed both in Brevibacillus choshinensis culture medium using an efficient promoter and the secretion signal of its surface layer protein, and in Escherichia coli cytoplasm with the amino-terminal His-tag (His-YkvV). His-YkvV was purified to homogeneity by Ni-NTA column. Both secreted YkvV and purified His-YkvV exhibited thiol-disulfide oxidoreductase activity.
- 社団法人 日本農芸化学会の論文
- 2004-08-23
著者
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HIRAI TAKANORI
Komaki Municipal Hospital
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Tanaka Ryoichi
Faculty Of Agriculture Kagoshima University
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Takagi H
Department Of Food Science And Technology Faculty Of Agriculture Nagoya University:(present Address)
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Takagi Hiroshi
Second Department Of Surgery Nagoya University
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MIYAUCHI Akira
Department of Surgery, Kuma Hospital
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Ishibashi Matsujiro
Faculty of Agri., Kagoshima Univ.
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Tokunaga Masao
Faculty of Agri., Kagoshima Univ.
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ARAKI Yoko
Faculty of Agriculture, Kagoshima University
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MIZUKAMI Makoto
Department of Research and Development, Higeta Shoyu Co.
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TOKUNAGA Hiroko
Faculty of Agriculture, Kagoshima University
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Takagi H
Department Of Bioscience Fukui Prefectural University
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Tokunaga M
Applied And Molecular Microbiology Faculty Of Agriculture Kagoshima University
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Tanaka Ryutaro
Faculty Of Agriculture Kagoshima University
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Takagi H
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.
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Miyauchi Akira
Department Of Orthopaedic Surgery Osaka Rosai Hospital
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Ishibashi Matsujiro
Applied And Molecular Microbiology Faculty Of Agriculture Kagoshima University
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Miyauchi Akira
Department Of Research And Development Higeta Shoyu Co.
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Mizukami M
Department Of Research And Development Higeta Shoyu Co.
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Mizukami Makoto
Department Of Food Science And Technology Faculty Of Agriculture Nagoya University:nihon Shokuhin Ka
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Araki Yoko
Faculty Of Agriculture Kagoshima University
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Tokunaga Masao
Faculty Of Agri. Kagoshima Univ.
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