Cloning and Nucleotide Sequence of the Maltopentaose-forming Amylase Gene from Pseudomonas sp. KO-8940
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概要
- 論文の詳細を見る
The gene coding for the maltopentaose-(G5)-forming amylase of Pseudomonas sp.KO-8940 was cloned into Escherichia coli and its nucleotides were sequenced. It was expected that a long open reading frame composed of 1,842-bp that encoded 614 amino acid residues for secretory precursor polypeptide including the typical signd sequence with an NH_2-terminal was the gene. An extract of Escherichia coli carrying the cloned G5-forming amylase gene had amylolytic activity with which produced only G5 from starch, the same as that of the donor strain enzyme. In the deduced primary structure of this enzyme, the four conserved regions of many α-amylases were found, and the COOH-terminal portion of this enzyme showed high homology with other raw starch digesting amylases.
- 社団法人日本農芸化学会の論文
- 1992-01-23
著者
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Takagi H
Department Of Food Science And Technology Faculty Of Agriculture Nagoya University:(present Address)
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KOBAYASHI Shoichi
National Food and Research Institute, Ministry of Agriculture, Forestry and Fisheries
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TAKAGI Hiroaki
Research Laboratory, Higeta Shoyu Co., Ltd.
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KADOWAKI Kiyoshi
Research Laboratory, Higeta Shoyu Co., Ltd.
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TAKANO Toshiya
National Food Research Institute
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Takagi H
Fukui Prefectural Univ. Fukui Jpn
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Kobayashi Shoichi
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries
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Kobayashi S
Department Of Food And Life-science Takasaki University Of Health And Welfare
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SHIDA Osamu
Research Laboratory, Higeta Shoyu Co., Ltd.
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Shida Osamu
Research Laboratory Higeta Shoyu Co. Ltd.
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Takano T
Calpis Co. Ltd. Kanagawa Jpn
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Kobayashi S
Fukui Univ. Technol. Fukui Jpn
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Kadowaki Kiyoshi
Research Laboratory Higeta Shoyu Co. Ltd.
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Takagi Hiroaki
Research Laboratory Higeta Shoyu Co. Ltd.
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