Purification and Chemical Properties of a Flocculant Produced by Paecilomyces
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概要
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A new flocculant for microbial cells was purified by ethanol precipitation and gel chromatography from the culture fluid of Paecilomyces sp. I-1. Isoelectric focusing of the flocculant (PF-101) showed a single band at pH 8.5, and its molecular weight was estimated to be over 300, 000 daltons by the ultra-filtration method. The results of elemental analysis, the IR spectrum and investigation of the acid hydrolysate by gas and liquid chromatography and colorimetric analysis suggested that PF-101 was a polysaccharide composed of galactosamine. About 80% of the galactosamine residues were N-unsubstituted and 8% were N-acetylated. Studies on deaminative cleavage, periodate oxidation and Smith degradation suggested that the galactosamine residues were mainly linked by α (1→4)-linkages.
- 社団法人 日本農芸化学会の論文
著者
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TAKAGI Hiroaki
Research Laboratory, Higeta Shoyu Co., Ltd.
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Kadowaki Kiyoshi
Research Laboratory Higeta Shoyu Co. Ltd.
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Takagi Hiroaki
Research Laboratory Higeta Shoyu Co. Ltd.
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