Molecular Cloning and Sequence Analysis of the Gene Encoding an Endo _α-1,4 Polygalactosaminidase of Pseudomonas sp. 881
スポンサーリンク
概要
- 論文の詳細を見る
The gene encoding an endoα-1,4 polygalactosaminidase (P-GalNase) from Pseudomonas sp. 881 was cloned into Escherichia coli DH1 and MV1184 by using charomid 9-36 and a pUC vector, and then sequenced. The gene encoding the enzyme P-GalNase consisted of 882 bp encoding 294 amino acid residues including a leader sequence. The deduced amino acid sequence did not show any extensive homology with sequences of other glycanases listed in the SWISS-PROT (GenBank) data bank, but we could observe some homology between the middle segment of the P-GalNase sequence and the conserved sequences of chitinases and related glycosidases.
- 社団法人日本生物工学会の論文
- 1995-10-25
著者
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IGARASHI Yasuo
Department of Biotechnology, The University of Tokyo
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KODAMA Tohru
Department of Textile Science and Technology, Shinshu University
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Tamura J
Research Laboratory Of Higeta Shoyu Co. Ltd.
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Tamura J
Tottori Univ. Tottori Jpn
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KADOWAKI Kiyoshi
Research Laboratory, Higeta Shoyu Co., Ltd.
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Tamura Jun-ichi
Research Laboratory Of Higeta Shoyu Co. Ltd.
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Igarashi Yasuo
Department Of Agricultural Chemistry The University Of Tokyo
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Igarashi Yasuo
Department Of Applied Biological Chemistry The University Of Tokyo
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Kodama Tohru
Department Of Agricultural Chemistry The University Of Tokyo
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HASEGAWA KANAME
Research Laboratory of Higeta Shoyu Co. Ltd.
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Tamura Jun-ichi
Research Laboratory Higeta Shoyu Co. Ltd.
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Kadowaki Kiyoshi
Research Laboratory Higeta Shoyu Co. Ltd.
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Kodama Tohru
Department Of Applied Biological Chemistry The University Of Tokyo
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Kodama Tohru
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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