The Mode of Action of Endo α-1,4 Polygalactosaminidase from Pseudomonas sp. 881 on Galactosaminooligosaccharides
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概要
- 論文の詳細を見る
The mode of action of the endo α-1,4 polygalactosaminidase from Pseudomonas sp. 881 on galactosaminooligosaccharides (GOSs) was studied. The enzyme could hydrolyze α-1,4 polygalactosamine to GOSs by the endo-split manner. Tetraose and longer GOSs were hydrolyzed to galactosaminobiose and galactosaminotriose as the final products. Galactosaminomonomer (galactosamine) could not be produced as an enzymatic product. From the dependency of kinetic parameters on the chain lengths of the substrates, it was suggested that the enzyme has 8 subsites. A catalytic site of the enzyme is located between the third and the fourth sites from the non-reducing end, since the main product from GOSs was galactosaminotriose, and galactosaminotetraitol remained in the hydrolyzate of galactosaminoheptaitol digestion. The enzyme showed transglycosylating activity on GOS4.
- 社団法人日本農芸化学会の論文
- 1992-03-23
著者
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Tamura J
Research Laboratory Of Higeta Shoyu Co. Ltd.
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Tamura J
Tottori Univ. Tottori Jpn
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KADOWAKI Kiyoshi
Research Laboratory, Higeta Shoyu Co., Ltd.
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Tamura Jun-ichi
Research Laboratory Of Higeta Shoyu Co. Ltd.
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Abe T
Niigata Univ. Niigata Jpn
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HASEGAWA KANAME
Research Laboratory of Higeta Shoyu Co. Ltd.
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Tamura Jun-ichi
Research Laboratory Higeta Shoyu Co. Ltd.
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ABE Toshiro
Research Laboratory of Higeta Shoyu Co., Ltd.
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Abe Toshiro
Research Department Matsushita Electric Works Ltd.
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Kadowaki Kiyoshi
Research Laboratory Higeta Shoyu Co. Ltd.
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