Isolation and Characterization of Predominant Microorganisms during Decomposition of Waste Materials in a Field-Scale Composter

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概要

• 論文の詳細を見る

• A self-heating field-scale composter treating agro-industrial wastes within a period of 30 d was analyzed by denaturing gradient gel electrophoresis (DGGE) (Pedro et al., J. Biosci. Bioeng., 91, 159-165, 2001). Three major bands were derived from Propionibacterium acnes, Methylobacterium mesophilicum or M. radiotolerans, and Bacillus thermocloacae. Strains MSP09A and MSP06G with close affiliation to P, acnes and B, thermocloacae, respectively, were successfully isolated. Based on quantitative-PCR results, the relative population of MSP09A increased towards the end of the composting process (mesophilic stage) while MSP06G seemed to predominate during the middle period (thermophilic stage). These results correlated highly with their growth temperatures. MSP09A and MSP06G had different metabolic profiles which were largely affected by culture conditions. MSP09A was able to utilize large complex molecules of lipids and proteins. An interspecies relationship in terms of metabolites such as propionic acid was expected between the two microorganisms.

• 社団法人日本生物工学会の論文
• 2003-04-25

著者

• ISHII Masaharu

  Department of Biotechnology, The University of Tokyo

• IGARASHI Yasuo

  Department of Biotechnology, The University of Tokyo

• 石井 正浩

  University Of Tokyo

• 石井 正治

  東京大学大学院農学生命科学研究科応用工学専攻

• Igarashi Y

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Igarashi Yasuo

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences University Of Tokyo

• Igarashi Yasuo

  University Of Tokyo

• PEDRO MANNIX

  Department of Biotechnology, Graduate School of Agricultural and Life Sciences, the University of To

• HARUTA SHIN

  Department of Biotechnology, Graduate School of Agricultural and Life Sciences, the University of To

• Ishii M

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences University Of Tokyo

• Ishii Masaharu

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences Univ. Of Tokyo

• Igarashi Y

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sci. The Univ. Of Tokyo Yayoi 1-1-1 B

• NAKAMURA KOHEI

  Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of To

• HAZAKA MASARU

  Kennan Eisei Kogyo Co.,Hazaka Plant Laboratory

• Haruta Shin

  Division Of Microbial Chemistry Faculty Of Pharmaceutical Science Chiba University

• Ishii Masaharu

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Igarashi Yasuo

  Department Of Biotechnology The University Of Tokyo

• Nakamura K

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Hazaka Masaru

  Kennan Eisei Kogyo Co.

• Pedro Mannix

  Department Of Biotechnology Graduate School Of Agricultural And Life Science The University Of Tokyo

• Igarashi Y

  Univ. Tokyo Tokyo Jpn

• Igarashi Yasuo

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Nakamura Kohei

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Igarashi Yasuo

  Department Of Agricultural Chemistry The University Of Tokyo

• Nakamura Kazunori

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Ishii Masaharu

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Haruta Shin

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Hasegawa S

  Department Of Biomolecular Engineering Graduate School Of Engineering Thhoku University

• Haruta Shin

  Department Of Biological Sciences Tokyo Metropolitan University

• PEDRO MANNIX

  Department of Biotechnology Graduate School of Agricultural and Life Sciences,The University of Tokyo:(Present address)National Institute of Molecular Biology and Biotechnology,University of the Philippines at Los Bafios,College

• ISHII MASAHARU

  Department of Biotechnology Graduate School of Agricultural and Life Sciences,The University of Tokyo

• NAKAMURA KOHEI

  Department of Biotechnology Graduate School of Agricultural and Life Sciences,The University of Tokyo

• ISHII Masaharu

  Department of Agricultural Chemistry, The University of Tokyo

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