Carboxylation Reactions of Pyruvate:Ferredoxin Oxidoreductase and 2-Oxoglutarate:Ferredoxin Oxidoreductase from Hydrogenobacter thermophilus TK-6
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概要
- 論文の詳細を見る
Enzymatic reactions involving pyruvate : ferredoxin oxidoreductase and 2-oxoglutarate : ferredoxin oxidoreductase from a thermophilic, aerobic, chemolithoautotrophic, and hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus TK-6, were investigated as the CO_2 exchange reaction and CO_2 fixation reaction using ferredoxin isolated from the same organism as a reductant. The reduced ferredoxin was required in the pyruvate synthetic reaction and the 2-oxoglutarate synthetic reaction by a cell extract that had been treated with a PD-10 column to remove the low molecular weight substances. [^<14>C] Pyruvate and [^<14>C] 2-oxoglutarate were detected as products of pyruvate synthetic and 2-oxoglutarate synthetic reactions, respectively. Further evidence for the operation of pyruvate : ferredoxin oxidoreductase and 2-oxoglutarate : ferredoxin oxidoreductase was obtained from experiments on CO_2 exchange reactions using the purified enzymes.
- 社団法人日本農芸化学会の論文
- 1997-03-23
著者
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ISHII Masaharu
Department of Biotechnology, The University of Tokyo
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IGARASHI Yasuo
Department of Biotechnology, The University of Tokyo
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石井 正浩
University Of Tokyo
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Igarashi Y
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Igarashi Yasuo
University Of Tokyo
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YOON Ki-Seok
Department of Biotechnology, The University of Tokyo
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KODAMA Tohru
Department of Textile Science and Technology, Shinshu University
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Ishii Masaharu
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences Univ. Of Tokyo
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Igarashi Y
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sci. The Univ. Of Tokyo Yayoi 1-1-1 B
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Ishii Masaharu
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Yoon Ki
Department Of Biotechnology The University Of Tokyo
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Igarashi Y
Univ. Tokyo Tokyo Jpn
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Yokoyama Keiichi
Department Of Pathology And Cell Regulation Kyoto Prefectural University Of Medicine
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Yokoyama Keiichi
Department Of Biotechnology Graduate School Of Agricultre And Life Science The University Of Tokyo
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Yokoyama Keiichi
Food Research & Development Laboratories
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Igarashi Yasuo
Department Of Agricultural Chemistry The University Of Tokyo
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Kodama T
Department Of Biotechnology The University Of Tokyo:(present Address)faculty Of Textile Science And
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Kodama Tohru
Department Of Agricultural Chemistry The University Of Tokyo
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Kokubo Tetsuro
Department Of Biotechnology The University Of Tokyo:(present Address)division Of Gene Function In An
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Yokoyama K
Ajinomoto Co. Inc. Kawasaki Jpn
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YOKOYAMA Keiichi
Institute of Life Sciences, Ajinomoto Co., Inc.
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Yokoyama K
Central Research Laboratories Ajinomoto Co. Inc.
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Yokoyama Keiichi
京都府立医科大学 第2病理
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Kodama Tohru
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Yokoyama Keiichi
Institute Of Life Sciences Ajinomoto Co. Inc.
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ISHII Masaharu
Department of Agricultural Chemistry, The University of Tokyo
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