O_2-stable membrane-bound [NiFe]hydrogenase from a newly isolated Citrobacter sp. S-77(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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Hydrogenases are of great interest due to their potential use in H_2-based technology. However, most hydrogenases are highly sensitive to O_2, which have been the major bottleneck in hydrogenase studies. Here we report an O_2-stable membrane-bound [NiFe]hydrogenase (MBH) purified from a newly isolated strain, S-77. According to the 16S rRNA gene sequence and phylogenetic analysis of the strain S-77, it belongs to the genus of Citrobacter. In vitro experiments using the cytoplasmic membrane of strain S-77 suggested that a cytochrome b acts as the physiological electron acceptor of the MBH. The purified MBH was composed of a dimer of heterodimers, consisting of two distinct subunits with the molecular weights of 58.5 and 38.5 kDa. The enzyme showed a specific activity for H_2-oxidation of 661 U/mg, which is 35-fold greater than that for H_2-production of 18.7 U/mg. Notably, the MBH showed a remarkable O_2-stability, maintaining almost 95% of its original activity even after incubation for 30 h in air at 4℃. These results suggest that the O_2-stable MBH may play an important role in the H_2-metabolic pathway under the aerobic conditions of Citrobacter sp. S-77. This is the first report of the purification and biochemical characterization of an O_2-stable MBH from the genus of Citrobacter.