Cloning and Expression of a Clostridium cellobioparum Cellulase Gene and Its Excretion from Escherichia coil JM109
スポンサーリンク
概要
- 論文の詳細を見る
A cellulase gene of Clostridium cellobioparum was cloned on vector pUC19 to form a recombinant plasmid pCMC and expressed in Escherichia coli. The expressed enzyme showed carboxymethylcellulase activity but could not degrade Avicel. E. coli JM109 carrying pCMC1 excreted a large amount of cellulase into a culture medium. The C. cellobioparum DNA sequence in the pCMC1 directed the cellulase excretion.
- 社団法人日本生物工学会の論文
- 1989-08-25
著者
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IGARASHI Yasuo
Department of Biotechnology, The University of Tokyo
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Kato Junichi
Department Of Cardiology Asahikawa Medical College
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KODAMA Tohru
Department of Textile Science and Technology, Shinshu University
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Igarashi Yasuo
Department Of Agricultural Chemistry The University Of Tokyo
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Igarashi Yasuo
Department Of Agricultural Chemistry University Of Tokyo
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Kodama Tohru
Department Of Agricultural Chemistry University Of Tokyo
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Kato Junichi
Department Of Agricultural Biological Chemistry College Of Bioresource Sciences Nihon University
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SHIMA SEIGO
Department of Biology, Abiko Research Laboratory, Central Research Institute of Electric Power Indus
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Kato Junichi
Department Of Agricultural Chemistry University Of Tokyo
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Shima Seigo
Max-planck-insititut Fur Terrestrische Mikrobiologie And Laboratorium Fur Mikrobiologie Fachbereich
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Shima Seigo
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Kodama Tohru
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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