ATP Amplification for Ultrasensitive Bioluminescence Assay : Detection of a Single Bacterial Cell
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概要
- 論文の詳細を見る
We developed an ultrasensitive bioluminescence assay of ATP by employing (i) adenylate kinase (ADK) for converting AMP+ATP to two molecules of ADP, (ii) polyphosphate (polyP) kinase (PPK) for converting ADP back to ATP (ATP amplification), and (iii) a commercially available firefly luciferase. A highly purified PPK-ADK fusion protein efficiently amplified ATP, resulting in high levels of bioluminescence in the firefly luciferase reaction. The present method, which was approximately 10,000-fold more sensitive to ATP than the conventional bioluminescence assay, allowed us to detect bacterial contamination as low as one colony-forming unit (CFU) of Escherichia coli per assay.
- 社団法人 日本農芸化学会の論文
- 2004-06-23
著者
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Ohtake Hisao
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Kato Junichi
Department Of Cardiology Asahikawa Medical College
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KURODA Akio
Department of Molecular Biotechnology, Hiroshima University
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Ohtake H
Department Of Biotechnology Osaka University
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Ohtake Hisao
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Ohtake H
Institute Of Applied Microbiology University Of Tokyo
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Ohtake Hisao
Institute Of Applied Microbiology The University Of Tokyo
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Ohtake Hisao
Department Of Fermentation Technology Hiroshima University
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Kato J
Department Of Molecular Biotechnology Hiroshima University
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TAKIGUCHI NOBORU
Department of Fermentation Technology, Hiroshima University
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SATOH Tetsuya
Department of Applied Chemistry, Faculty of Engineering, Osaka University
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Satoh Tetsuya
Department Of Applied Chemistry Faculty Of Engineering Osaka University
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Kuroda A
Department Of Molecular Biotechnology Hiroshima University
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Kuroda Akio
Department Of Molecular Biotechnology Hiroshima University
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Kato Junichi
Department Of Agricultural Biological Chemistry College Of Bioresource Sciences Nihon University
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Kato J
Department Of Chemical Engineering Faculty Of Engineering Shizuoka University
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Takiguchi Noboru
Department Of Molecular Biotechnology Hiroshima University
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Takiguchi Noboru
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kato Junichi
Department Of Agricultural Chemistry University Of Tokyo
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Kuroda Akio
Department Of Chemistry Faculty Of Science Nagoya University
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Satoh Tetsuya
Department Of Molecular Biotechnology Graduate School Of Advanced Sciences Of Matter Hiroshima Unive
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