Overexpression of GADD34 Enhances Production of Recombinant Human Antithrombin III in Chinese Hamster Ovary Cells(CELL AND TISSUE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
To improve the production of recombinant human antithrombin III (AT III) in Chinese hamster ovary (CHO) cells, the gene encoding growth arrest and DNA damage inducible protein 34 (GADD34), which is a transcription factor involved in the unfolded protein response (UPR), was cloned from CHO-K1 cells. Overexpression of GADD34 significantly enhanced the production of recombinant AT-III in CHO 13D-35D cells. The specific rate of AT-III production in the GADD34-overexpressing CHO 13D-35D cells reached approximately 28pg/cell/d. After 144h of incubation, the AT-III concentration in the culture supernatant was approximately 40% higher than that observed in the case of the parental CHO 13D-35D cells. The mRNA expression, specific activity, and fucosylation of AT-III were not affected by GADD34 overexpression. Overexpression of GADD34 is a promising method of improving the production of secreted protein pharmaceuticals in CHO cells.
- 社団法人日本生物工学会の論文
- 2008-12-25
著者
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Honda Kohsuke
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Omasa Takeshi
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Ohtake Hisao
Department of Biotechnology, Graduate School of Engineering, Osaka University
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大政 健史
大阪大学大学院工学研究科
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Omasa Takeshi
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Omasa Takeshi
大阪大学生物工学国際交流センター
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Takami Takashi
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Ohya Tomoshi
Mitsubishi Tanabe Pharma Corporation
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Ohya Tomoshi
Protein Research Laboratory, Mitsubishi Tanabe Pharma Corporation
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Kiyama Eriko
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Hayashi Tetsuji
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Nishii Hiroko
Protein Research Laboratory, Mitsubishi Tanabe Pharma Corporation
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Miki Hideo
Protein Research Laboratory, Mitsubishi Tanabe Pharma Corporation
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Kobayashi Kaoru
Protein Research Laboratory, Mitsubishi Tanabe Pharma Corporation
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Nishii Hiroko
Protein Research Laboratory Mitsubishi Tanabe Pharma Corporation
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Nishii Hiroko
大阪大学 工学研究科生命先端工学専攻
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Kiyama Eriko
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Kiyama Eriko
大阪大学 工学研究科生命先端工学専攻
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Ohtake H
Department Of Biotechnology Osaka University
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Ohtake Hisao
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Ohtake H
Institute Of Applied Microbiology University Of Tokyo
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Ohtake Hisao
Institute Of Applied Microbiology The University Of Tokyo
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Ohya Tomoshi
Protein Research Laboratory Pharmaceutical Research Division Mitsubishi Pharma Corporation
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Honda Kohsuke
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Honda Kohsuke
Grad. Sch. Of Eng. Osaka Univ.
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Honda Kohsuke
大阪大学 工学研究科生命先端工学専攻
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Ohtake Hisao
Department Of Fermentation Technology Hiroshima University
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Takami Takashi
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Okumura H
Electrotechnical Lab. Ibaraki Jpn
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Ohtake Hisao
大阪大学 工学研究科生命先端工学専攻
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Kobayashi Kaoru
Protein Research Laboratory Pharmaceutical Research Division Mitsubishi Pharma Corporation
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Miki Hideo
Protein Res. Lab. Mitsubishi Tanabe Pharma Corp.
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Miki Hideo
Protein Research Laboratory Mitsubishi Tanabe Pharma Corporation
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Hayashi Tetsuji
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Omasa Takeshi
Department Of Biological Science And Technology Institute Of Technology And Science The University O
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Kobayashi Kaoru
Protein Research Laboratory Mitsubishi Tanabe Pharma Corporation
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Omasa Takeshi
Department Of Biological Science And Technology Biosystems Engineering Institute Of Technology And Science The University Of Tokushima
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Ohya Tomoshi
Protein Research Laboratory Mitsubishi Tanabe Pharma Corporation
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Okumura Hajime
Department of Biotechnology, Graduate School of Engineering, Osaka University
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