Different Properties of Gene Products of Three Sets Ribulose 1, 5-Bisphosphate Carboxylase/Oxygenase from a Marine Obligately Autotrophic Hydrogen-Oxidizing Bacterium, Hydrogenovibrio marinus Strain MH-110

スポンサーリンク

概要

• 論文の詳細を見る

• Hydrogenovibrio marinus strain MH-110 is an obligately lithoautotrophic hydrogen-oxidizing hacterium, possessing three sets of the genes for ribulose 1, 5-bisphosphate carboxylase/oxygenase(RubisCO); one form II type(L_x)and two form I type(L_8S_8)enzymes. The genes for the form I type enzymes are named cbbLS-1 and cbbLS-2, and that for the form II type enzyme is named cbbM. These three sets of genes were cloned ill plasmid vectors, and the expression of the genes in E. coli cells were studied. The three RubisCOs were purified through the same steps, And their specificity factors(τ Values)were measured. The τ values of CbbLS-1 and CbbLS-2 were higher than that of CbbM, but lower than those of other form I RubisCOs. The specific activity of CbbM was very low and CbbM was inactivated easily during the process of purification. Immunochemical analyses revealed that the antibody against form I type reacted only with CbbL-1 and CbbL-2, and the antibody against form II type reacted only with CbbM. The antibody to neither form of RubisCO demonstrated cross-reactivity for the other form.

• 社団法人日本生物工学会の論文
• 1998-02-25

著者

• IGARASHI Yasuo

  Department of Biotechnology, The University of Tokyo

• Igarashi Y

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Igarashi Yasuo

  University Of Tokyo

• KODAMA TOHRU

  Faculty of Textile Science and Technology, Shinshu University

• HAYASHI NOBUHIRO

  Department of Biotechnology, University of Tokyo

• Igarashi Y

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sci. The Univ. Of Tokyo Yayoi 1-1-1 B

• Chung Seon

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research (riken)

• Chung S

  Department Of Biotechnology University Of Tokyo

• Igarashi Y

  Univ. Tokyo Tokyo Jpn

• NISHIHARA HIROFUMI

  Department of Agricultural Chemistry, The University of Tokyo

• Nishihara Hirofumi

  Department Of Applied Biological Resource Science Ibaraki University

• Igarashi Yasuo

  Department Of Agricultural Chemistry The University Of Tokyo

• Kodama T

  Department Of Biotechnology The University Of Tokyo:(present Address)faculty Of Textile Science And

• Kokubo Tetsuro

  Department Of Biotechnology The University Of Tokyo:(present Address)division Of Gene Function In An

• OGUNI ASAKO

  Department of Biotechnology, University of Tokyo

• YAGUCHI TOSHIAKI

  Institute for Fundamental Research, Suntory Limited

• CHUNG SEONG-YONG

  Department of Biotechnology, University of Tokyo

• Nishihara H

  Department Of Bioresource Sciences School Of Agriculture Ibaraki University

• Hayashi N

  Department Of Biotechnology University Of Tokyo

• Oguni Asako

  Department Of Biotechnology University Of Tokyo

• Hayashi Nobuhiro

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Yaguchi Toshiaki

  Institute For Fundamental Research Suntory Limited

関連論文

• Gene Structure and Expression Profile of Cytochrome bc Nitric Oxide Reductase from Hydrogenobacter thermophilus TK-6
• Purification, Characterization, and Gene Cloning of Thermophilic Cytochrome cd_1 Nitrite Reductase from Hydrogenobacter thermophilus TK-6(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Effect of adding carbon fiber textiles to methanogenic bioreactors used to treat an artificial garbage slurry(ENVIRONMENTAL BIOTECHNOLOGY)
• BR-P7 Succinate production from glycerol with acetate as an electron sink(Section IV Biorefinery)
• Sequencing and Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Analysis of Four Hydrogenase Gene Clusters from an Obligately Autotrophic Hydrogen-Oxidizing Bacterium, Hydrogenobacter thermophilus TK-6(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEE
• Purification and Properties of Glutamine Synthetase from Hydrogenobacter thermophilus TK-6(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Two Tandemly Arranged Ferredoxin Genes in the Hydrogenobacter thermophilus Genome : Comparative Characterization of the Recombinant [4Fe-4S] Ferredoxins
• Analyses of Microbial Community within a Composter Operated Using Household Garbage with Special Reference to the Addition of Soybean Oil
• Purification and Characterization of Membrane-bound Hydrogenase from Hydrogenobacter thermophilus Strain TK-6,an Obligately Autotrophic, Thermophilic, Hydrogen-oxidizing Bacterium
• Growth Characteristics and Dense Culture of a Thermophilic Cyanobacterium, Chroococcidiopsis sp. Strain TS-821
• Carboxylation Reactions of Pyruvate:Ferredoxin Oxidoreductase and 2-Oxoglutarate:Ferredoxin Oxidoreductase from Hydrogenobacter thermophilus TK-6
• Purification and Characterization of Ferredoxin from Hydrogenobacter thermophilus Strain TK-6
• Purification and Some Properties of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase from a Thermophilic Hydrogen-Oxidizing Bacterium, Pseudomonas hydrogeneothermophila Strain TH-1
• Methionaquinone is a Direct Natural Electron Acceptor for the Membrane-bound Hydrogenase in Hydrogenobacter thermophilus Strain TK-6(Biological Chemistry)
• Colony Formation of Hydrogenobacter thermophilus on a Plate Solidified with GELRITE(Microbiology & Fermentation Industry)
• Purification and Some Properties of Cytochrome c552 from Hydrogenobacter thermophilus
• The Membrane-bound Hydrogenase Reduces Cytochrome c552 in Hydrogenobacter thermophilus strain TK-6
• Microbial Diversity in Biodegradation and Reutilization Processes of Garbage
• Cloning and Characterization of the Genes Involved in the Degradation of Dibenzofuran by Terrabacter sp. Strain DBF63
• Cloning and Nucleotide Sequences of the Genes Involved in the meta-Cleavage Pathywa of Cumene Degradation in Pseudomonas fluorescens IP01
• Cloning, Nucleotide Sequence, and Characterization of the Genes Encoding Enzymes Involved in the Degradation of Cumene to 2-Hydroxy-6-Oxo-7-Methylocta-2,4-Dienoic Acid in Pseudomonas fluorescens IP01
• Culture-Independent Phylogenetic Analysis of the Microbial Community in Industrial Sugarcane Bagasse Feedstock Piles
• Effect of Adding Cellulolytic Bacterium on Stable Cellulose-Degrading Microbial Community(ENVIRONMENTAL BIOTECHNOLOGY)
• PA-36 The Thermodynamic of Interspecies Electron Transfer(Symbiosis/Cell to cell interaction,Poster presentation A)
• 2G14-3 Comparisons of Sensory Quality, Nutrition Value and Microbial Composition among Home-made and Commercial Soypaste in China
• Archaeal Population on Supporting Material in Methanogenic Packed-Bed Reactor(ENVIRONMENTAL BIOTECHNOLOGY)
• 3I09-3 Development of a microbial community being effective for ensiling rice straw at low temperature
• Microbial Community in Methanogenic Packed-Bed Reactor Successfully Operating at Short Hydraulic Retention Time(ENVIRONMENTAL BIOTECHNOLOGY)
• Effects of Water-Soluble Carbohydrate Content on Silage Fermentation of Wheat Straw(ENVIRONMENTAL BIOTECHNOLOGY)
• Succession of a Microbial Community during Stable Operation of a Semi-continuous Garbage-Decomposing System(ENVIRONMENTAL BIOTECHNOLOGY)
• Isolation and Characterization of Predominant Microorganisms during Decomposition of Waste Materials in a Field-Scale Composter
• Characterization of a Microorganism Isolated from the Effluent of Hydrogen Fermentation by Microflora
• 119 Selective and sensitive detection of Escherichia coil in cornposting process by PCR amplification of the 16S rRNA genes
• Denaturing Gradient Gel Electrophoresis Analyses of Microbial Community from Field-Scale Composter
• Physiological and Chemotaxonomical Studies on Microflora within a Composter Operated at High Temperature
• Occurrence of Hydrogen-Oxidizing Ralstonia Species as Primary Microorganisms in the Mt. Pinatubo Volcanic Mudflow Deposits(Soil Biology)
• Purification and Characterization of Membrane-bound Hydrogenase from a Thermophilic Hydrogen-Oxidizing Bacterium, Pseudomonas hydrogenothermophila Strain TH-1
• Isolation and Cultivation of Thermophilic Cyanobacteria from Hot Springs of Northern Thailand
• Electron Transport System in Pseudomonas hydrogenothermophila Strain TH-1, a Thermophilic Hydrogen-oxidizing Bacterium
• Cloning and Sequence of the L_2 Form of RubisCO from a Marine Obligately Autotrophic Hydrogen-oxidizing Bacterium, Hydrogenovibrio marinus Strain MH-110
• Production and Properties of Glycogen in the Marine Obligate Chemolithoautotroph, Hydrogenovibrio marinus
• Growth Characteristisc and High Cell-Density Cultivation of a Marine Obligately Chemolithoautotrophic Hydrogen-Oxidizing Bacterium Hydrogenovibrio marinus Strain MH-110 under a Continuous Gas-Flow System
• Thermostability of Pseudomonas hydrogenothermophila Cytochrome c-552
• The Role of the nirQOP Genes in Energy Conservation during Anaerobic Growth of Pseudomonas aeruginosa
• Production of Recombinant Cytochrome c-551 in a Pseudomonas aeruginosa Mutant Strain
• Structure and ANR-Dependent Transcription of the nir Genes for Denitritication from Pseudomonas aeruginosa
• Siccanin Rediscovered as a Species-Selective Succinate Dehydrogenase Inhibitor
• Heterologous Expression of Hydrogenobacter thermophilus Cytochrome c-552 in the Periplasm of Pseudomonas aeruginosa
• Responses of Transformed Root Culture of Atropa belladonna to Salicylic Acid Stress
• Promotive Effect of Auxins on UDP-Glucose : Flavonol Glucosyltransferase Activity in Vitis sp.Cell Cultures
• A Comparison of GUS Activity after Liquid- and Air-Heat Shock Treatments in Transgenic Nicotiana plumbaginifolia Harboring the Arabidopsis HSP18.2 Promoter-GUS Chimeric Gene
• Suppressed Phenylalanine Ammonia-Lyase Activity after Heat Shock in Transgenic Nicotiana plumbaginifolia Containing an Arabidopsis HSP18.2-Parsley PAL2 Chimera Gene
• Effects of Aeration on Tropane Alkaloid Production by Transformed Root of Atropa belladonna in Flask Cultures
• Methanogenic pathway and community structure in a thermophilic anaerobic digestion process of organic solid waste(ENVIRONMENTAL BIOTECHNOLOGY)
• Different Properties of Gene Products of Three Sets Ribulose 1, 5-Bisphosphate Carboxylase/Oxygenase from a Marine Obligately Autotrophic Hydrogen-Oxidizing Bacterium, Hydrogenovibrio marinus Strain MH-110
• Cloning, Sequence and Overexpression of the Thermophilic Cyanobacterium Gene for the Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase
• Purification of RuBisCO from the Thermophilic Cyanobacterium Synechococcus sp. Strain a-1
• Random Amplified Polymorphic DNA (RAPD) Analyses for Discriminating Genotypes of Microcystis Cyanobacteria
• Construction of Novel Expression Vectors Effective in Pseudomonas Cells(Microbiology & Fermentation Industry)
• Isolation and Characterization of CO_2-Fixing Hydrogen-Oxidizing Marine Bacteria
• Cloning and Expression of a Clostridium cellobioparum Cellulase Gene and Its Excretion from Escherichia coil JM109
• Need for Cytochrome bc_1 Complex for Dissimilatory Nitrite Reduction of Pseudomonas aeruginosa(Microbiology & Fermentation Technology)
• Acetoacetyl-CoA Transferase and Thiolase Reactions Are Catalyzed by a Single Enzyme in Clostridium acetobutylicum ATCC824(Microbiology & Fermentation Industry)
• Molecular Cloning and Sequence Analysis of the Gene Encoding an Endo _α-1,4 Polygalactosaminidase of Pseudomonas sp. 881
• 469 Evolution-assisted Design of A Novel Cytochrome c with Enhanced Thermostability
• Structure of Ribulose 1,5-bisphosphate Carboxylase / Oxygenase Gene Cluster from a Thermophilic Hydrogen-Oxidizing Bacterium, Hydrogenophilus thermoluteolus, and Phylogeny of the Fructose 1,6-Bisphosphate Aldolase Encoded by cbbA in the Cluster
• Identification and Characterization of Phycobiliprotein from a Thermophilic Cyanobacterium, Chroococcidiopsis sp. Strain TS-821
• A Non-radioisotopic Anion-exchange Chromatographic Method to Measure the CO_2/O_2 Specificity Factor for Ribulose Bisphosphate Carboxylase/Oxygenase
• Effect of Carbon Cioxide Concentration on the Growth and RubisCO Activity of a Thermophilic Cyanobacterium, Chroococcidiopsis sp. Strain TS-821
• Methanogenic communities on the electrodes of bioelectrochemical reactors without membranes(ENVIRONMENTAL BIOTECHNOLOGY)
• Does Reconstruction of Posterior Ligamentous Complex with Extensor Musculature Decrease Axial Symptoms After Cervical Laminoplasty?
• Determinative Factors of Competitive Advantage between Aerobic Bacteria for Niches at the Air-Liquid Interface
• N-myristoylated proteins, key components in intracellular signal transduction systems enabling rapid and flexible cell responses
• Changes in Size of Intracellular Pools of Coenzyme A and Its Thioesters in Escherichia coli K-12 Cells to Various Carbon Sources and Stresses
• Determinative Factors of Competitive Advantage Between Aerobic Bacteria for Niches at the Air-Liquid Interface
• Thiosulfate Oxidation by a Thermo-Neutrophilic Hydrogen-Oxidizing Bacterium, Hydrogenobacter thermophilus
• Quantitative and Specific Detection of a Trichloroethylene-degrading Methanotroph, Methylocystis sp. Strain M, by a Most Probable Number-Polymerase Chain Reaction Method(Microbiology & Fermentation Technology)
• Identification of the Plasmid-Encoded 4,5-Dihydro-4,5-Dihydroxyphthalate Dehydrogenase Gene of Pseudomonas testosteroni Involved in Phthalate Metabolism
• Analysis of the Molecular Species of Hydrogenase in the Cells of an Obligately Chemolithoautotrophic, Marine Hydrogen-Oxidizing Bacterium, Hydrogenovibrio marinus(Microbiology & Fermentation Technology)
• Methanogenic pathway and community structure in a thermophilic anaerobic digestion process of organic solid waste
• Culture-Independent Phylogenetic Analysis of the Microbial Community in Industrial Sugarcane Bagasse Feedstock Piles
• Detecting weak protein-protein interactions by modified far-western blotting(METHODS)
• Changes in the gene expression profile of Acetobacter aceti during growth on ethanol(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Detecting weak protein-protein interactions by modified far-western blotting
• Construction of hydrogen fermentation from garbage slurry using the membrane free bioelectrochemical system(ENVIRONMENTAL BIOTECHNOLOGY)
• Involvement of Flagella-Driven Motility and Pili in Pseudomonas aeruginosa Colonization at the Air-Liquid Interface
• Involvement of Flagella-Driven Motility and Pili in Pseudomonas aeruginosa Colonization at the Air-Liquid Interface
• Role of the glyoxylate pathway in acetic acid production by Acetobacter aceti(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Construction of hydrogen fermentation from garbage slurry using the membrane free bioelectrochemical system
• Transcriptome Analyses of Metabolic Enzymes in Thiosulfate- and Hydrogen-Grown Hydrogenobacter thermophilus Cells
• Candidatus Methanogranum caenicola: a Novel Methanogen from the Anaerobic Digested Sludge, and Proposal of Methanomassiliicoccaceae fam. nov. and Methanomassiliicoccales ord. nov., for a Methanogenic Lineage of the Class Thermoplasmata
• Identification of the Genes Encoding Nitric Oxide Reductase in the Aerobic Photosynthetic Bacterium Roseobacter denitrificans OCh114
• The Growth of Oxygen-resistant Hydrogen Bacterium N34 in a Jar Fermentor under High Oxygen Tension and Stability of the Oxyhydrogen Reaction
• Effect of Organic Compoundson the Growth of an Obligately Autotrophic Hydrogen-Oxidizing Bacterium, Hydrogenobacter thermophilus TK-6
• The Deficient Carbohydrate Metabolic Pathways and the Incomplete Tricarboxylic Acid Cycle in an Obligately Autotrophic Hydrogen-oxidizing Bacterium
• Excretion of L-Tryptophan by Analogue-resistant Mutants of Pseudomonas hydrogenothermophila TH-1 in Autotrophic Cultures
• 2-Methylthio-1, 4-naphthoquinone, a New Quinone from an Extremely Thermophilic Hydrogen Bacterium
• Role of the glyoxylate pathway in acetic acid production by Acetobacter aceti
• Candidatus Methanogranum caenicola : a Novel Methanogen from the Anaerobic Digested Sludge, and Proposal of Methanomassiliicoccaceae fam. nov. and Methanomassiliicoccales ord. nov., for a Methanogenic Lineage of the Class Thermoplasmata
• Inhibitory Effects of Ferrihydrite on a Thermophilic Methanogenic Community

もっと見る 閉じる

スポンサーリンク