Identification of the Plasmid-Encoded 4,5-Dihydro-4,5-Dihydroxyphthalate Dehydrogenase Gene of Pseudomonas testosteroni Involved in Phthalate Metabolism

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概要

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• THe 4,5-dihydro-4,5-dihydroxyphathalate (DDP)-accumulating Tn5-inserted mutant strain M4-122 of Pseudomonas testosteroni M4-1 was assumed to have a Tn5 insertion at the DDP dehydrogenase gene. We cloned the EcoRI fragment from total DNA of the wild strain corresponding to the Tn5 insertion site of mutant strain M4-122 to verify the existence of the DDP dehydrogenase gene and the arrangement of other genes involved in phthalate metabolism. As a result, the DDP dehydrogenase gene was proven to be located downstream of the 4,5-dihydroxyphthalate decarboxylase gene and the genes were incorporated in a plasmid.

• 公益社団法人日本生物工学会の論文
• 1994-12-25

著者

• OMORI TOSHIO

  Biotechnology Research Center, The University of Tokyo

• Omori Toshio

  Biotechnoiogy Researchcenter The University Of Tokyo

• Omori T

  Univ. Tokyo Tokyo Jpn

• Lee J‐h

  Korea Res. Inst. Biosci And Biotechnol. Taejon Kor

• Kodama T

  Department Of Biotechnology The University Of Tokyo:(present Address)faculty Of Textile Science And

• Kokubo Tetsuro

  Department Of Biotechnology The University Of Tokyo:(present Address)division Of Gene Function In An

• LEE Jong-Hoon

  Department of Materials Science and Engineering, Seoul National University

• KODAMA TOHRU

  Deaprtment of Agricultural Chemistry, The University of Tokyo

• LEE JONG-HOON

  Department of Agricultural Chemistry, The University of Tokyo

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