Identificaton of the Metabolic Intermediates of Phathalate by Tn5 Mutants of Pseudomonas testosteroni and Analysis of the 4,5-Dihydroxyphthalate Decarboxylase Gene

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概要

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• Phthalate-, 4,5-dihydro-4,5-dihydroxyphthalate (DDP)-, 4,5-dihydroxyphthalate (DHP)-, and protocatechuate-accumulating mutants of Pseudomonas testosteroni M4-1 were isolated by transposon insertion mutagenesis. From the DDP-accumulating, transposon-inserted mutant strain M4-122,the 4,5-dihydro-xyphthalate decarboxylase gene which is involved in phthalate metabolism was cloned and its nucleotide sequence determined. The structural gene, designated phtD, was 990 bp, corresponding to a protein of 330 amino acid residues (calculated molecular weight 37,200). The phtD gene was expressed in Escherichia coli by its own promoter located upstream of the phtD open reading frame. The promoter shows high homology with the E. coli σ^<70> consensus sequence. The putative amino acid sequence has 77.6% homology with that of the pht5 gene from Pseudomonas putida. The nucleotide sequence of the upstream region of the phtD gene has high homology with that of pht1,the putative positive regulator for the other pht genes of P. putida. The deduced amino acid sequence of the upstream region of the phtD gene also shows high similarity with that of Pht1. From these results, the genes of P. testosteroni and P. putida involved in phthalate metabolism would seem to have evolved from the same origin but diversified to compose different gene orders in different organisms.

• 公益社団法人日本生物工学会の論文
• 1994-06-25

著者

• OMORI TOSHIO

  Biotechnology Research Center, The University of Tokyo

• Omori Toshio

  Biotechnoiogy Researchcenter The University Of Tokyo

• Omori T

  Univ. Tokyo Tokyo Jpn

• Lee J‐h

  Korea Res. Inst. Biosci And Biotechnol. Taejon Kor

• Kodama T

  Department Of Biotechnology The University Of Tokyo:(present Address)faculty Of Textile Science And

• Kodama Tohru

  Department Of Agricultural Chemistry The University Of Tokyo

• Kokubo Tetsuro

  Department Of Biotechnology The University Of Tokyo:(present Address)division Of Gene Function In An

• LEE Jong-Hoon

  Department of Materials Science and Engineering, Seoul National University

• Kodama Tohru

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• LEE JONG-HOON

  Department of Agricultural Chemistry, The University of Tokyo

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