Oxidation of Trichloroethylene and Dimethyl Sulfide by a Marine Methylomicrobium Strain Containing Soluble Methane Monooxygenase
スポンサーリンク
概要
- 論文の詳細を見る
Sixteen marine methanotrophic bacteria were isolated and 14 marine methanotrophic mixed cultures were obtained. They were assayed for soluble methane monooxygenase (sMMO) by naphthalene oxidation and only one isolate (strain NI) was positive. Strain NI degraded trichloroehylene (TCE) more efficiently than other methanotrophic isolates containing no sMMO only under copper limiting conditions. Dimethyl sulfide (DMS), one of the radiatively important trace gases released from the sea, was transformed to dimethyl sulfoxide (DMSO) by methanotrophs and the efficiency for the transformation of DMS to DMSO was not as much affected by the presence of sMMO as that of TCE. The taxonomical properties of strain NI and phylogenetic analysis based on 16S rDNA genes indicated that strain NI was a type I methanotroph belonging to the genus Methylomicrobium, and closely related to Methylomicrobium pelagicum. The partial mmoX gene of strain NI was amplified by the primers common to three other mmoX genes and its 270 bp were sequenced. 77 residues out of the 89 amino acids derived from the sequences were common among the four mmoX genes.
- 社団法人日本農芸化学会の論文
- 1998-10-23
著者
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OMORI TOSHIO
Biotechnology Research Center, The University of Tokyo
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Fuse Hiroyuki
Institute For Marine Resources And Environment National Institute Of Advanced Industrial Science And
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Omori Toshio
Biotechnology Research Center The University Of Tokyo
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Omori Toshio
Biotechnoiogy Researchcenter The University Of Tokyo
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FUSE Hiroyuki
Chugoku National Industrial Research Institute
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TAKIMURA Osamu
Chugoku National Industrial Research Institute
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MURAKAMI Katsuji
Chugoku National Industrial Research Institute
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YAMAOKA Yukiho
Chugoku National Industrial Research Institute
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OHTA Misaki
Towa Kagaku Co., Ltd.
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INOUE Hiroyuki
Chugoku National Industrial Research Institute
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OCLARIT JoseM.
Iligan Institute of Technology, Mindanao State University
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Takimura Osamu
Institute For Marine Resources And Environment National Institute Of Advanced Industrial Science And
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Ohta Misaki
Towa Kagaku Co. Ltd.
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Murakami K
Institute For Marine Resources And Environment National Institute Of Advanced Industrial Science And
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Yamaoka Y
National Inst. Advanced Industrial Sci. And Technol. Hiroshima Jpn
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Oclarit Josem.
Iligan Institute Of Technology Mindanao State University
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Inoue H
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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