Structural Determination and Functional Analysis of a Soluble Matrix Protein Associated with Calcification of the : Exoskeleton of the Crayfish, Procambarus clarkii
スポンサーリンク
概要
- 論文の詳細を見る
A novel matrix protein named calcification-associated soluble protein-2 (Casp-2) was isolated from the acetic acid-soluble fraction of the exoskeleton of the crayfish Procambarus clarkii, and its primary structure was determined by a combination of peptide sequencing, mass spectral analysis, and cDNA cloning. Casp-2 consists of 117 amino acid residues and has a chitin-binding consensus sequence, the so-called Rebers-Riddiford (R-R) consensus sequence. Casp-2 exhibited an inhibitory activity on calcium carbonate precipitation from its supersaturated solution in vitro, suggesting association with calcification of the exoskeleton. Reverse transcription PCR and Northern blot analyses indicated that the Casp-2 gene was expressed only at the epidermis throughout the molting stages, and most strongly at the late pre-molt stage. Recombinant Casp-2 showed weak affinity to chitin in spite of having the R-R consensus sequence. These results indicate that Casp-2 interacts loosely with chitin fibrils and regulates calcification in the cuticle.
- 社団法人 日本農芸化学会の論文
- 2008-10-23
著者
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Suzuki Masakazu
Dep. Of Biology Fac. Of Sci. Shizuoka Univ.
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SUZUKI Michio
Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The Universit
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Nagasawa Hiromichi
Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Nagasawa Hiromichi
Department Of Applied Biological Chemistry Graduate School Of Agriculture And Life Sciences Universi
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Nagasawa Hiromichi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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YUASA-HASHIMOTO Naomi
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Inoue H
Department Of Applied Biological Chemistry The University Of Tokyo
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Inoue Hirotaka
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Yuasa Hashimoto
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Suzuki M
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Inoue H
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Suzuki Michio
Department Of Electronics Faculty Of Engineering Hokkaido University
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Suzuki Michio
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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