Cloning and expression of a cDNA encoding an insoluble matrix protein in the gastroliths of a crayfish, Procambarus clarkii
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概要
- 論文の詳細を見る
In the crayfish Procambarus clarkii, the gastroliths are formed as a paired structure in the stomach during the premolt period, and contain calcium carbonate and a small amount of an organic matrix. In this investigation, a cDNA encoding an insoluble matrix protein was isolated from P. clarkii. The open reading frame encoded 505 amino acid residues including two unique repeated sequences. The N-terminal half of the amino acid sequence, which included 10-amino-acid repeats, exhibited a high degree of similarity to that of involucrin, a protein synthesized in human keratinocytes. Northern blot analysis revealed that mRNA encoding the matrix protein is specifically expressed in the gastrolith discs during the premolt period in which the gastroliths formed. In the gastrolith discs, levels of expression of this mRNA correlated increases in weights of the gastroliths concomitant with their formation. Organ culture of the gastrolith discs suggested that expression of mRNA in the discs is induced by molting hormone, 20-hydroxyecdysone. These results reinforced the relationship between the matrix protein and formation of the gastroliths. Functional analysis showed that the protein inhibits calcium carbonate crystallization in a solution system, suggesting that the protein plays a role in the calcification of the gastroliths.
- 社団法人日本動物学会の論文
- 1999-08-15
著者
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Takagi Yasuaki
Otsuchi Marine Research Center Ocean Research Institute The University Of Tokyo
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WATANABE Tatsuo
School of Food and Nutritional Sciences, University of Shizuoka
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Nagasawa Hiromichi
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Ishii K
Hokkaido Univ. Hokkaido Jpn
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Tsutsui Naoaki
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Tsutsui Naoaki
Department Of Molecular Biology Ocean Research Institute The University Of Tokyo:department Of Appli
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Watanabe Toshiki
Department Of Marine Bioscience Ocean Research Institute The University Of Tokyo
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Yanagisawa T
Utsunomiya Univ. Tochigi Jpn
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Watanabe Tatsuo
Graduate School Of Nutritional And Environmental Sciences And Coe Program In The 21st Century Univ.
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Watanabe T
Department Of Bioscience And Biotechnology Graduate School Of Agriculture Kinki University
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Ishii Katsuaki
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Watanabe Tetsuro
Department Of Bioresources Chemistry Chiba University
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Nagasawa Hiromichi
Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Nagasawa Hiromichi
Department Of Applied Biological Chemistry Graduate School Of Agriculture And Life Sciences Universi
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Nagasawa Hiromichi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Takagi Y
Department Of Biology Faculty Of Science Nara Women's University
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Ishii K
Transformation Laboratory Department Of Molecular And Cell Biology Forestry And Forest Products Rese
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WATANABE Toshiro
Fukuoka Agricultural Research Center
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Watanabe Toshiki
Department Of Cardiovascular Surgery Fukushima Medical University School Of Medicine
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