Molecular cloning and expression analysis of a cDNAs encoding androgenic gland hormone precursors from two Porcellionidae species, Porcellio scaber and P. dilatatus
スポンサーリンク
概要
- 論文の詳細を見る
Male sexual characteristics in Crustacea are induced by androgenic gland hormone (AGH), which is produced by the male-specific androgenic gland. Recently, AGH in the terrestrial isopod Armadillidium vulgare was characterized and its cDNA cloned, the first example in which the structure of AGH was elucidated. We report here the molecular cloning of cDNAs encoding AGH precursors from two additional terrestrial isopods, Porcellio scaber and P. dilatatus. cDNA fragments encoding Porcellio scaber AGH (Pos-AGH) and P. dilatatus AGH (Pod-AGH) were amplified by RT-PCR using degenerate oligonucleotide primers designed based on the amino acid sequence of A. vulgare AGH (Arv-AGH). Subsequently, full length cDNAs were obtained by 5'- and 3'-RACE. Both AGH precursors consisted of a signal peptide, B chain, C peptide and A chain, and exhibited the same organization as that of Arv-AGH. The amino acid sequences of the A and B chains, which comprise mature AGH peptide, were highly conserved among the three species, while that of the C peptide showed only low sequence similarity. In Northern blot analysis, each cDNA fragment used as a probe specifically hybridized with a single band (0.75 kb) in mRNA extracted from whole male reproductive organs. In analysis of the tissue-specific gene expression of these two AGHs by RT-PCR, it was revealed that both AGH transcripts were detected only in cDNA synthesized using total RNA from the testis carrying the androgenic glands, but not in that from testis only, seminal vesicle, vas deferens, or hepatopancreas.
- 社団法人日本動物学会の論文
- 2003-01-25
著者
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Nagasawa Hiromichi
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Ohira T
Kanagawa Univ. Kanagawa Jpn
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Ohira Tsuyoshi
Faculty Of Science Kanagawa University
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Ohira Tsuyoshi
Laboratory Of Genome Science Graduate School Of Marine Science And Technology Tokyo University Of Ma
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Ohira Tsuyoshi
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Ohira Tsuyoshi
Graduate School Of Agricultural And Life Sciences University Of Tokyo
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OKUNO Atsuro
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Hasegawa Yuriko
Department of Biology, Keio University
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Tominaga Satoshi
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences The Un
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Ohira Tsuyoshi
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Nagasawa Hiromichi
Department Of Applied Biological Chemistry Graduate School Of Agriculture And Life Sciences Universi
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Nagasawa Hiromichi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Okuno Atsuro
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Hasegawa Y
Department Of Biology Keio University
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Hasegawa Yuriko
Department Of Biology Keio University
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Tominaga S
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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長澤 寛道
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Nagasawa Hiromichi
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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