Preparation of two recombinant crustacean hyperglycemic hormones from the giant freshwater prawn Macrobrachium rosenbergii, and their hyperglycemic activities
スポンサーリンク
概要
- 論文の詳細を見る
Crustacean hyperglycemic hormone (CHH) is released from the X-organ/sinus gland complex located in the eyestalks, and regulates glucose levels in the hemolymph. In the giant freshwater prawn (Macrobrachium rosenbergii), two cDNAs encoding different CHH molecules were previously cloned by other workers. One of these (Mar-CHH-2) was expressed only in the eyestalks, whereas the other (Mar-CHH-L) was expressed in the heart, gills, antennal gland, and thoracic ganglion, but not in the eyestalks. However, their biological activities had not yet been characterized. Therefore, in this study, recombinant Mar-CHH-2 (rMar-CHH-2) and Mar-CHH-L (rMar-CHH-L) were produced using an E. coli expression system, by expression in bacterial cells and recovery in the insoluble fraction. Thereafter, rMar-CHH-2 and rMar-CHH-L were subjected to refolding and were subsequently purified by reversed-phase HPLC. The rMar-CHH-2 and rMar-CHH-L thus obtained exhibited the same disulfide bond arrangements as those of other CHHs reported previously, indicative of natural conformation. In in vivo bioassay, rMar-CHH-2 showed significant hyperglycemic activity, whereas rMar-CHH-L had no effect. These results indicate that Mar-CHH-L does not function as a CHH, but may have some other, unknown function.
- 2006-04-25
著者
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WILDER Marcy
Japan International Research Center for Agricultural Sciences
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Tsutsui Naoaki
Japan International Research Center for Agricultural Sciences
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Ohira Tsuyoshi
Japan International Research Center for Agricultural Sciences
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Wilder M
Japan International Research Center For Agricultural Sciences
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Wilder Marcy
Department Of Fisheries Faculty Of Agriculture The University Of Tokyo
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Wilder Marcy
Japan International Research Center For Agricultural Sciences Ministry Of Agriculture Forestry And F
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Nagasawa Hiromichi
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Ohira T
Kanagawa Univ. Kanagawa Jpn
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Ohira Tsuyoshi
Faculty Of Science Kanagawa University
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Ohira Tsuyoshi
Laboratory Of Genome Science Graduate School Of Marine Science And Technology Tokyo University Of Ma
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Tsutsui Naoaki
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Tsutsui Naoaki
Department Of Molecular Biology Ocean Research Institute The University Of Tokyo:department Of Appli
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Ohira Tsuyoshi
Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Ohira Tsuyoshi
Japan Int'l Research Center For Agricultural Sciences
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Ohira Tsuyoshi
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Nagasawa Hiromichi
Department Of Applied Biological Chemistry Graduate School Of Agriculture And Life Sciences Universi
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Nagasawa Hiromichi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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長澤 寛道
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Wilder Marcy
Japan International Res. Center For Agricultural Sci. Ibaraki Jpn
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Wilder Marcy
Japan Int'l Research Center For Agricultural Sciences
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Nagasawa Hiromichi
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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