Purification and Structural Determination of a Phosphorylated Peptide with Anti-calcification and Chitin-binding Activities in the Exoskeleton of the Crayfish, Procambarus clarkii(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
Organic matrices in calcified hard tissues have been considered to control calcification. A matrix peptide, designated CAP-1, was extracted and purified by anion-exchange and reverse-phase high performance liquid chromatographies from the exoskeleton of the crayfish, Procambarus clarkii. The amino acid sequence of CAP-1 was determined by mass spectral and sequence analyses of the intact peptide and its enzymatically digested peptides. CAP-1 consisted of 78 amino acid residues, including a phosphoserine residue, and was rich in acidic amino acid residues. CAP-1 had a Rebers-Riddiford consensus sequence, which is conserved in cuticle proteins from many arthropods. CAP-1 inhibited precipitation of calcium carbonate in an in vitro anti-calcification assay dose-dependently, and completely inhibited it at 3×10^<-7>м. CAP-1 also showed chitin-binding ability, indicating that this molecule was bifunctional and played an important role in formation of the exoskeleton.
- 社団法人日本農芸化学会の論文
- 2001-08-23
著者
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Nagasawa Hiromichi
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Ozaki Noriaki
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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INOUE Hirotaka
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyushu University
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Nagasawa Hiromichi
Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Nagasawa Hiromichi
Department Of Applied Biological Chemistry Graduate School Of Agriculture And Life Sciences Universi
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Nagasawa Hiromichi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Ozaki N
Department Of Marine Bioscience The University Of Tokyo
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Ozaki Noriaki
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Inoue Hirotaka
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Inoue H
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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