A monoclonal antibody against a synthetic carboxyl-terminal fragment of the eclosion hormone of the silkworm, Bombyx mori : characterization and application to immunohistochemistry and affinity chromatography : Immunology
スポンサーリンク
概要
- 論文の詳細を見る
Monoclonal antibodies were produced using as antigen a synthetic fragment correspond-ing to the C-terminal portion of the eclosion hormone (EH) of the silkworm, Bombyx mori. The characterization of these antibodies using ELISA revealed that one of them recognized specifically both the synthetic fragment and native eclosion hormone. Immunohistochemistry using this antibody indicated that EH was produced in two pairs of median neurosecretory cells of the brain. Affinity chromatography of a partially purified EH using a column on which this antibody had been immobilized showed that EH activity was completely adsorbed to this column and eluted with the synthetic fragment with about 60% recovery.
- 社団法人日本動物学会の論文
- 1990-02-15
著者
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Suzuki A
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Suzuki Atsushi
Basic Research Department Prima Meat Packers Co. Ltd.
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Nagasawa Hiromichi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Suzuki A
Department Of Obstetrics And Gynecology Keio University School Of Medicine
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Fugo H
Tokyo Univ. Agriculture And Technol. Tokyo Jpn
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Mizoguchi Akira
Department Of Anatomy And Neurobiology Kyoto University Graduate School Of Medicine.
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ISHIZAKI Hironori
Department of Pathology, Kurume University School of Medicine
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Mizoguchi A
Nagoya Univ. Aichi Jpn
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KONO Takaharu
Department of Agricultural Chemistry, The University of Tokyo
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石崎 宏矩
Nagoya Univ.
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MIZOGUCHI Akira
Biological Institute, Faculty of Science, Nagoya University
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NAGASAWA Hironichi
Department of Agricidtural Chemistry, Faculty of Agriculture, The University of Tokyo
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FUGO HAJIME
Biological Institute, Faculty of Science, Nagoya University
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Suzuki Akinori
Tokyo University of Agriculture and Technology
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Kono Takaharu
Department Of Agricultural Chemistry The University Of Tokyo
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Suzuki Akinori
Chemistry Faculty Of Agriculture The University Of Tokyo
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Ishizaki Hironori
Department Of Pathology Kurume University School Of Medicine
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Kono Takaharu
Department Of Agricidtural Chemistry Faculty Of Agriculture The University Of Tokyo
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Suzuki A
Faculty Of Agriculture The University Of Tokyo
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