Subcellular Localization of Galactosyltransferase Associated with Tumors in Endometrial and Ovarian Cancer Cells
スポンサーリンク
概要
- 論文の詳細を見る
Frozen or resin sections of normal human endometrium, endometrial adenocarcinoma, and ovarian adenocarcinoma were examined with two monoclonal antibodies for galactosyltransferase associated with tumor (GAT) by immunofluorescence and immunoelectron microscopy. One antibody (MAb8628) stained the trans-cisternae of the Golgi apparatus, the trans-Golgi network, and the intracytoplasmic vesicles more intensely in cancer cells than in normal endometrial glandular cells. The other antibody (MAb8513) stained intracytoplasmic vesicles more intensely in cancer cells than in normal cells. MAb8513 also showed moderate staining of the trans-cisternae of the Golgi apparatus and trans-Golgi network in cancer cells, but only faint staining of these structures in normal glandular cells, indicating that GAT was overexpressed in the cancer cells. An immunofluorescence study using serial semithin cryosections (1 μm) demonstrated that the staining pattern of each antibody was different inside a single cancer cell, conforming to the two patterns mentioned above. The reason for this difference in staining remains unclear. GAT is a soluble form of (β1,4-galactosyltransferase, so a difference in the cleavage site of the membrane-bound peptide may cause changes in immunoreactivity after its conversion to the soluble form (i.e., GAT) in endometrial or ovarian adenocarcinoma cells.
- 日本組織細胞化学会の論文
著者
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Aoki Daisuke
Department of Obstetrics and Gynecology, School of Medicine, Keio University
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NAKAMURA Seigo
Breast Center
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NOZAWA Shiro
Department of Obstetrics and Gynecology, School of Medicine, Keio University
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Suzuki Atsushi
Department of Cardiology, Musashino Red Cross Hospital
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Kawakami Hayato
Department of Anatomy, Kyorin University School of Medicine
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HIRANO Hiroshi
Department of Anatomy, Kyorin University School of Medicine
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Nozawa S
Department Of Obstetrics And Gynecology Keio University School Of Medicine
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Nozawa Shiro
Department Of Obstetrics And Gynecology Keio School Of Medicine
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Suzuki Nao
Department Of Obstetrics And Gynecology School Of Medicine Keio University
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Suzuki Nao
Department Of General Dentistry Fukuoka Dental College
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SUSUMU Nobuyuki
Department of Obstetrics and Gynecology, School of Medicine, Keio University
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Aoki Daisuke
Department Of Obstetrics And Gynecology School Of Medicine Keio University
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Aoki Daisuke
Department Of Aeronautics And Space Engineering Tohoku University
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Suzuki Atsushi
Biodevelopment Division Central Institute Nagoya Seiraku Co. Ltd.
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Suzuki Atsushi
Department Of Applied Biological Chemistry Faculty Of Agriculture University Of Niigata
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Suzuki Atsushi
Department Of Animal Husbandry Faculty Of Agriculture Tohoku University
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Suzuki Atsushi
Synthetic Crystal Research Laboratory Faculty Of Engineering Nagoya University
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Suzuki Atsushi
Department Of Pathology Obstetrics And Gynecology Keio University School Of Medicine
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Suzuki Atsushi
Basic Research Department Prima Meat Packers Co. Ltd.
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Suzuki Atsushi
Department Of Applied Biochemistry Faculty Of Agriculture University Of Niigata
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Kawakami Hayato
Second Dep. Of Anatomy Dep. Of Biochemistry Kyorin Univ. School Of Medicine
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Kawakami Hayato
Dept. Of Anatomy Kyorin University School Of Medicine
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Kawakami Hayato
Department Of 2nd Anatomy Kyorin University School Of Medicine
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Suzuki A
Department Of Obstetrics And Gynecology Keio University School Of Medicine
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Hirano Hiroshi
Food Research Lab. Toyama Food Research Institute
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Hirano Hiroshi
Second Department Of Pathology Hyogo College Of Medicine
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Hirano H
Department Of Anatomy Kyorin University School Of Medicine
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Uejima Takao
Environment and Safety Dept., Konica Co. Ltd.
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Kawakami H
Department Of Anatomy Kyorin University School Of Medicine
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Uejima Takao
Environment And Safety Dept. Konica Co. Ltd.
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Nozawa Shiro
Breast Center
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Hirano H
Univ. Hyogo Hyogo
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Aoki Daiyu
Department Of Obstetrics And Gynecology Keio University School Of Medicine
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Aoki Daiya
Departments Of Histology And Cell Biology Nagasaki Univ. Schol Of Medicine:departments Of Urology Na
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Hirano Hiroshi
Department Of Anatomy Kansai Medical School
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Hirano Hiroshi
Department Of 2nd Anatomy Kyorin University School Of Medicine
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