ESTABLISHMENT OF HUMAN UTERINE CERVICAL CANCER CELL LINE AND COMPARATIVE STUDIES BETWEEN NORMAL AND MALIGNANT UTERINE CERVICAL CELLS IN VITRO

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The cell lines designated SKG-2 and SKG-2B were established from human uterine cervical cancer (epidermoid carcinoma) before and after Bleomycin treatment. The SKG-2B cell line was subcultivated more than 45 times within 14 months. The cultured cells appeared epithelial in shape, with a pavement-like arrangement and grew in multi-layers without contact inhibition. The cytology revealed anaplastic and pleomorphic features. In electron microscopy, the cells were characterized by smooth nuclear membranes, multiple large nucleoli, numerous microvilli, desmosomal cell contacts and few tonofibrils. The chromosome studie showed aneuploidy and the modal chromosomal number was stable in the hypo-diploid range. Thc marker, a large submetacentric chromosome, was constantly present. The SKG-2B cells were transplanted subcutaneously to nude mice and submucousally to immunedepressed hamster cheek pouches, and produced tumor which resembled the original tumor. No differences between SKG-2 and SKG-2B could be identified in the growth and morphological characteristics. On the contrary, the normal uterine cervical squamous epithelial cells in vitro were uniform in shape and growth, and showed a limitted life span. The cells showed a favorable outgrowth from the explant and cornification in the growing process. The cells could not be subcultivated and eventually degenerated after maintaining the culture for 2 months.
社団法人日本産科婦人科学会の論文
1978-07-01

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