Effects of Heat and High-Pressure Treatments on Antigenicity of Beef Extract(Food & Nutrition Science)
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概要
- 論文の詳細を見る
The sera of bovine gamma globulin (BGG) positive beef allergic patients were used in this study in order to investigate changes in IgE-specific binding activity with regard to beef extract altered by heat or high-pressure treatment. In inhibition-ELISA, the sample treated at 60℃ did not show any significant changes in the antigenicity of BGG, but the sample treated at 100℃ showed a decrease of the antigenicity. In the case of the treatment with heating at 100℃, heat-coagulation occurred in the beef extract. The resulting supernatant and precipitate of the sample by centrifugation were analyzed by immunoblotting. Only the fraction of precipitate showed a specific binding activity with the sera. Based on this result, it was speculated that the persistent antigenicity found even after the treatment at 100℃ in inhibition-ELISA remained principally in the heat-coagulated fraction, which indicated the importance of the method of handling the heat-coagulation in heat treatment. High-pressure treatments (200 Mpa-600 Mpa) of beef extract did not show any significant changes in the binding with the sera.
- 社団法人日本農芸化学会の論文
- 2002-01-23
著者
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Suzuki Atsushi
Department of Cardiology, Musashino Red Cross Hospital
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Suzuki Atsushi
Biodevelopment Division Central Institute Nagoya Seiraku Co. Ltd.
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Suzuki Atsushi
Department Of Applied Biological Chemistry Faculty Of Agriculture University Of Niigata
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Suzuki Atsushi
Department Of Animal Husbandry Faculty Of Agriculture Tohoku University
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Suzuki Atsushi
Synthetic Crystal Research Laboratory Faculty Of Engineering Nagoya University
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Suzuki Atsushi
Department Of Pathology Obstetrics And Gynecology Keio University School Of Medicine
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Suzuki Atsushi
Basic Research Department Prima Meat Packers Co. Ltd.
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Suzuki Atsushi
Department Of Applied Biochemistry Faculty Of Agriculture University Of Niigata
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Suzuki A
Department Of Obstetrics And Gynecology Keio University School Of Medicine
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Suzuki A
Department Of Applied Biological Chemistry Faculty Of Agriculture University Of Niigata
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HAN Gi
Doctor's Program in Functional Biology, Graduate School of Science and Technology, University of Nii
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MATSUNO Masatomo
Department of Pediatrics, Yoshida Hospital
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IKEUCHI Yoshihide
Division of Food Animal Science, Graduate School of Agricultural Sciences, Kyushu University
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Han Gidong
Doctor's Program In Functional Biology Graduate School Of Science And Technology University Of
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Ikeuchi Y
Division Of Food Animal Science Graduate School Of Agricultural Sciences Kyushu University
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Matsuno Masatomo
Department Of Pediatrics Yoshida Hospital
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